Immortalization of Human B Cells: Analysis of B Cell Repertoire and Production of Human Monoclonal Antibodies

One of the major challenges in human B cell immunology field has been the objective to establish stable monoclonal cells lines that express the B cell receptor (BCR) on their cell surface and secrete antibodies. Such a system is extremely attractive not only for studying various aspect of BCR signal...

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Detalles Bibliográficos
Autor principal: Traggiai, Elisabetta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119954/
https://www.ncbi.nlm.nih.gov/pubmed/22723100
http://dx.doi.org/10.1007/978-1-61779-931-0_10
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author Traggiai, Elisabetta
author_facet Traggiai, Elisabetta
author_sort Traggiai, Elisabetta
collection PubMed
description One of the major challenges in human B cell immunology field has been the objective to establish stable monoclonal cells lines that express the B cell receptor (BCR) on their cell surface and secrete antibodies. Such a system is extremely attractive not only for studying various aspect of BCR signaling but also for the generation of human monoclonal antibody and the analysis of the human B cell repertoire. This chapter describes an efficient method to immortalize and clone human B cells by Epstein–Barr Virus (EBV) transformation.
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spelling pubmed-71199542020-04-06 Immortalization of Human B Cells: Analysis of B Cell Repertoire and Production of Human Monoclonal Antibodies Traggiai, Elisabetta Antibody Methods and Protocols Article One of the major challenges in human B cell immunology field has been the objective to establish stable monoclonal cells lines that express the B cell receptor (BCR) on their cell surface and secrete antibodies. Such a system is extremely attractive not only for studying various aspect of BCR signaling but also for the generation of human monoclonal antibody and the analysis of the human B cell repertoire. This chapter describes an efficient method to immortalize and clone human B cells by Epstein–Barr Virus (EBV) transformation. 2012-05-23 /pmc/articles/PMC7119954/ /pubmed/22723100 http://dx.doi.org/10.1007/978-1-61779-931-0_10 Text en © Springer Science+Business Media, LLC 2012 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Traggiai, Elisabetta
Immortalization of Human B Cells: Analysis of B Cell Repertoire and Production of Human Monoclonal Antibodies
title Immortalization of Human B Cells: Analysis of B Cell Repertoire and Production of Human Monoclonal Antibodies
title_full Immortalization of Human B Cells: Analysis of B Cell Repertoire and Production of Human Monoclonal Antibodies
title_fullStr Immortalization of Human B Cells: Analysis of B Cell Repertoire and Production of Human Monoclonal Antibodies
title_full_unstemmed Immortalization of Human B Cells: Analysis of B Cell Repertoire and Production of Human Monoclonal Antibodies
title_short Immortalization of Human B Cells: Analysis of B Cell Repertoire and Production of Human Monoclonal Antibodies
title_sort immortalization of human b cells: analysis of b cell repertoire and production of human monoclonal antibodies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7119954/
https://www.ncbi.nlm.nih.gov/pubmed/22723100
http://dx.doi.org/10.1007/978-1-61779-931-0_10
work_keys_str_mv AT traggiaielisabetta immortalizationofhumanbcellsanalysisofbcellrepertoireandproductionofhumanmonoclonalantibodies

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