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Mapping the Protein–Protein Interactome Networks Using Yeast Two-Hybrid Screens
The yeast two-hybrid system (Y2H) is a powerful method to identify binary protein–protein interactions in vivo. Here we describe Y2H screening strategies that use defined libraries of open reading frames (ORFs) and cDNA libraries. The array-based Y2H system is well suited for interactome studies of...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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2015
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7120425/ https://www.ncbi.nlm.nih.gov/pubmed/26621469 http://dx.doi.org/10.1007/978-3-319-23603-2_11 |