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Multiprotein Complex Production in Insect Cells by Using Polyproteins

A powerful approach utilizing polyproteins for balancing stoichiometry of recombinant multiprotein complexes overproduced in baculovirus expression vector systems (BEVS) is described. This procedure has been implemented here in the MultiBac system but can also be directly adapted to all commonly use...

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Detalles Bibliográficos
Autores principales: Nie, Yan, Bellon-Echeverria, Itxaso, Trowitzsch, Simon, Bieniossek, Christoph, Berger, Imre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7120617/
https://www.ncbi.nlm.nih.gov/pubmed/24203328
http://dx.doi.org/10.1007/978-1-62703-691-7_8