Multiprotein Complex Production in Insect Cells by Using Polyproteins

A powerful approach utilizing polyproteins for balancing stoichiometry of recombinant multiprotein complexes overproduced in baculovirus expression vector systems (BEVS) is described. This procedure has been implemented here in the MultiBac system but can also be directly adapted to all commonly use...

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Autores principales: Nie, Yan, Bellon-Echeverria, Itxaso, Trowitzsch, Simon, Bieniossek, Christoph, Berger, Imre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7120617/
https://www.ncbi.nlm.nih.gov/pubmed/24203328
http://dx.doi.org/10.1007/978-1-62703-691-7_8
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author Nie, Yan
Bellon-Echeverria, Itxaso
Trowitzsch, Simon
Bieniossek, Christoph
Berger, Imre
author_facet Nie, Yan
Bellon-Echeverria, Itxaso
Trowitzsch, Simon
Bieniossek, Christoph
Berger, Imre
author_sort Nie, Yan
collection PubMed
description A powerful approach utilizing polyproteins for balancing stoichiometry of recombinant multiprotein complexes overproduced in baculovirus expression vector systems (BEVS) is described. This procedure has been implemented here in the MultiBac system but can also be directly adapted to all commonly used BEVS. The protocol details the design principles of polyprotein-expressing DNA constructs, the generation of composite baculovirus for polyprotein production, and the expression and in vivo processing of polyproteins in baculovirus infected insect cells.
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spelling pubmed-71206172020-04-06 Multiprotein Complex Production in Insect Cells by Using Polyproteins Nie, Yan Bellon-Echeverria, Itxaso Trowitzsch, Simon Bieniossek, Christoph Berger, Imre Structural Genomics Article A powerful approach utilizing polyproteins for balancing stoichiometry of recombinant multiprotein complexes overproduced in baculovirus expression vector systems (BEVS) is described. This procedure has been implemented here in the MultiBac system but can also be directly adapted to all commonly used BEVS. The protocol details the design principles of polyprotein-expressing DNA constructs, the generation of composite baculovirus for polyprotein production, and the expression and in vivo processing of polyproteins in baculovirus infected insect cells. 2013-07-27 /pmc/articles/PMC7120617/ /pubmed/24203328 http://dx.doi.org/10.1007/978-1-62703-691-7_8 Text en © Springer Science+Business Media, LLC 2014 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Nie, Yan
Bellon-Echeverria, Itxaso
Trowitzsch, Simon
Bieniossek, Christoph
Berger, Imre
Multiprotein Complex Production in Insect Cells by Using Polyproteins
title Multiprotein Complex Production in Insect Cells by Using Polyproteins
title_full Multiprotein Complex Production in Insect Cells by Using Polyproteins
title_fullStr Multiprotein Complex Production in Insect Cells by Using Polyproteins
title_full_unstemmed Multiprotein Complex Production in Insect Cells by Using Polyproteins
title_short Multiprotein Complex Production in Insect Cells by Using Polyproteins
title_sort multiprotein complex production in insect cells by using polyproteins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7120617/
https://www.ncbi.nlm.nih.gov/pubmed/24203328
http://dx.doi.org/10.1007/978-1-62703-691-7_8
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AT bieniossekchristoph multiproteincomplexproductionininsectcellsbyusingpolyproteins
AT bergerimre multiproteincomplexproductionininsectcellsbyusingpolyproteins

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