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Selection for 3′-End Triplets for Polymerase Chain Reaction Primers
Primer extension by thermostable DNA polymerase in PCR starts from the 3′-end of a primer. If the PCR starting process fails, the entire PCR fails. Primer sequences at the 3′-end often interfere with success in PCR experiments. Over 2000 primer sequences from successful PCR experiments used with var...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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2007
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7121268/ https://www.ncbi.nlm.nih.gov/pubmed/17951790 http://dx.doi.org/10.1007/978-1-59745-528-2_3 |