Sensitive Detection of SARS Coronavirus RNA by a Novel Asymmetric Multiplex Nested RT-PCR Amplification Coupled With Oligonucleotide Microarray Hybridization

We have developed a sensitive method for the detection of specific genes simultaneously. First, DNA was amplified by a novel asymmetric multiplex PCR with universal primer(s). Second, the 6-carboxytetramethylrhodamine (TAMRA)-labeled PCR products were hybridized specifically with oligonucleotide mic...

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Detalles Bibliográficos
Autores principales: Zhang, Zhi-wei, Zhou, Yi-ming, Zhang, Yan, Guo, Yong, Tao, Sheng-ce, Li, Ze, Zhang, Qiong, Cheng, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2005
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7122606/
https://www.ncbi.nlm.nih.gov/pubmed/16156097
http://dx.doi.org/10.1385/1-59259-923-0:59

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7122606/
https://www.ncbi.nlm.nih.gov/pubmed/16156097
http://dx.doi.org/10.1385/1-59259-923-0:59

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