In vitro and in vivo antimicrobial activity of two α-helical cathelicidin peptides and of their synthetic analogs

Two α-helical antimicrobial peptides (BMAP-27 and -28) and four synthetic analogs were compared for in vitro and in vivo antimicrobial efficacy. All peptides proved active in vitro at micromolar concentrations against a range of clinical isolates, including antibiotic-resistant strains. BMAP-27 and...

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Autores principales: Benincasa, Monica, Skerlavaj, Barbara, Gennaro, Renato, Pellegrini, Antonio, Zanetti, Margherita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2003
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7124310/
https://www.ncbi.nlm.nih.gov/pubmed/15019203
http://dx.doi.org/10.1016/j.peptides.2003.07.025
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author Benincasa, Monica
Skerlavaj, Barbara
Gennaro, Renato
Pellegrini, Antonio
Zanetti, Margherita
author_facet Benincasa, Monica
Skerlavaj, Barbara
Gennaro, Renato
Pellegrini, Antonio
Zanetti, Margherita
author_sort Benincasa, Monica
collection PubMed
description Two α-helical antimicrobial peptides (BMAP-27 and -28) and four synthetic analogs were compared for in vitro and in vivo antimicrobial efficacy. All peptides proved active in vitro at micromolar concentrations against a range of clinical isolates, including antibiotic-resistant strains. BMAP-27 and two analogs were more effective towards Gram-negative, and BMAP-28 towards Gram-positive organisms. In addition, BMAP-28 provided some protection in vitro against human herpes simplex virus type 1 (HSV-1). The parent peptides and mBMAP-28 analog protected mice from lethal i.p. infections in an acute peritonitis model at peptide doses significantly lower than those toxic to the animals, suggesting a satisfactory therapeutic index.
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spelling pubmed-71243102020-04-08 In vitro and in vivo antimicrobial activity of two α-helical cathelicidin peptides and of their synthetic analogs Benincasa, Monica Skerlavaj, Barbara Gennaro, Renato Pellegrini, Antonio Zanetti, Margherita Peptides Article Two α-helical antimicrobial peptides (BMAP-27 and -28) and four synthetic analogs were compared for in vitro and in vivo antimicrobial efficacy. All peptides proved active in vitro at micromolar concentrations against a range of clinical isolates, including antibiotic-resistant strains. BMAP-27 and two analogs were more effective towards Gram-negative, and BMAP-28 towards Gram-positive organisms. In addition, BMAP-28 provided some protection in vitro against human herpes simplex virus type 1 (HSV-1). The parent peptides and mBMAP-28 analog protected mice from lethal i.p. infections in an acute peritonitis model at peptide doses significantly lower than those toxic to the animals, suggesting a satisfactory therapeutic index. Elsevier Inc. 2003-11 2003-12-20 /pmc/articles/PMC7124310/ /pubmed/15019203 http://dx.doi.org/10.1016/j.peptides.2003.07.025 Text en Copyright © 2003 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Benincasa, Monica
Skerlavaj, Barbara
Gennaro, Renato
Pellegrini, Antonio
Zanetti, Margherita
In vitro and in vivo antimicrobial activity of two α-helical cathelicidin peptides and of their synthetic analogs
title In vitro and in vivo antimicrobial activity of two α-helical cathelicidin peptides and of their synthetic analogs
title_full In vitro and in vivo antimicrobial activity of two α-helical cathelicidin peptides and of their synthetic analogs
title_fullStr In vitro and in vivo antimicrobial activity of two α-helical cathelicidin peptides and of their synthetic analogs
title_full_unstemmed In vitro and in vivo antimicrobial activity of two α-helical cathelicidin peptides and of their synthetic analogs
title_short In vitro and in vivo antimicrobial activity of two α-helical cathelicidin peptides and of their synthetic analogs
title_sort in vitro and in vivo antimicrobial activity of two α-helical cathelicidin peptides and of their synthetic analogs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7124310/
https://www.ncbi.nlm.nih.gov/pubmed/15019203
http://dx.doi.org/10.1016/j.peptides.2003.07.025
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