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Improving Identification of In-organello Protein-Protein Interactions Using an Affinity-enrichable, Isotopically Coded, and Mass Spectrometry-cleavable Chemical Crosslinker

An experimental and computational approach for identification of protein-protein interactions by ex vivo chemical crosslinking and mass spectrometry (CLMS) has been developed that takes advantage of the specific characteristics of cyanurbiotindipropionylsuccinimide (CBDPS), an affinity-tagged isotop...

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Detalles Bibliográficos
Autores principales: Makepeace, Karl A. T., Mohammed, Yassene, Rudashevskaya, Elena L., Petrotchenko, Evgeniy V., Vögtle, F.-Nora, Meisinger, Chris, Sickmann, Albert, Borchers, Christoph H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Biochemistry and Molecular Biology 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7124466/
https://www.ncbi.nlm.nih.gov/pubmed/32051233
http://dx.doi.org/10.1074/mcp.RA119.001839