Cargando…
Taxogenomic assessment and genomic characterisation of Weissella cibaria strain 92 able to metabolise oligosaccharides derived from dietary fibres
The importance of the gut microbiota in human health has led to an increased interest to study probiotic bacteria. Fermented food is a source of already established probiotics, but it also offers an opportunity to discover new taxa. Four strains of Weissella sp. isolated from Indian fermented food h...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7125115/ https://www.ncbi.nlm.nih.gov/pubmed/32246087 http://dx.doi.org/10.1038/s41598-020-62610-x |
_version_ | 1783515880164950016 |
---|---|
author | Månberger, Anna Verbrugghe, Phebe Guðmundsdóttir, Elísabet Eik Santesson, Sara Nilsson, Anne Hreggviðsson, Guðmundur Óli Linares-Pastén, Javier A. Nordberg Karlsson, Eva |
author_facet | Månberger, Anna Verbrugghe, Phebe Guðmundsdóttir, Elísabet Eik Santesson, Sara Nilsson, Anne Hreggviðsson, Guðmundur Óli Linares-Pastén, Javier A. Nordberg Karlsson, Eva |
author_sort | Månberger, Anna |
collection | PubMed |
description | The importance of the gut microbiota in human health has led to an increased interest to study probiotic bacteria. Fermented food is a source of already established probiotics, but it also offers an opportunity to discover new taxa. Four strains of Weissella sp. isolated from Indian fermented food have been genome sequenced and classified into the species W. cibaria based on whole-genome phylogeny. The genome of W. cibaria strain 92, known to utilise xylooligosaccharides and produce lactate and acetate, was analysed to identify genes for oligosaccharide utilisation. Clusters including genes involved in transportation, hydrolysis and metabolism of xylooligosaccharides, arabinooligosaccharides and β-glucosides were identified. Growth on arabinobiose and laminaribiose was detected. A 6-phospho-β-glucosidase clustered with a phosphotransferase system was found upregulated during growth on laminaribiose, indicating a mechanism for laminaribiose utilisation. The genome of W. cibaria strain 92 harbours genes for utilising the phosphoketolase pathway for the production of both acetate and lactate from pentose and hexose sugars but lacks two genes necessary for utilising the pentose phosphate pathway. The ability of W. cibaria strain 92 to utilise several types of oligosaccharides derived from dietary fibres, and produce lactate and acetate makes it interesting as a probiotic candidate for further evaluation. |
format | Online Article Text |
id | pubmed-7125115 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-71251152020-04-08 Taxogenomic assessment and genomic characterisation of Weissella cibaria strain 92 able to metabolise oligosaccharides derived from dietary fibres Månberger, Anna Verbrugghe, Phebe Guðmundsdóttir, Elísabet Eik Santesson, Sara Nilsson, Anne Hreggviðsson, Guðmundur Óli Linares-Pastén, Javier A. Nordberg Karlsson, Eva Sci Rep Article The importance of the gut microbiota in human health has led to an increased interest to study probiotic bacteria. Fermented food is a source of already established probiotics, but it also offers an opportunity to discover new taxa. Four strains of Weissella sp. isolated from Indian fermented food have been genome sequenced and classified into the species W. cibaria based on whole-genome phylogeny. The genome of W. cibaria strain 92, known to utilise xylooligosaccharides and produce lactate and acetate, was analysed to identify genes for oligosaccharide utilisation. Clusters including genes involved in transportation, hydrolysis and metabolism of xylooligosaccharides, arabinooligosaccharides and β-glucosides were identified. Growth on arabinobiose and laminaribiose was detected. A 6-phospho-β-glucosidase clustered with a phosphotransferase system was found upregulated during growth on laminaribiose, indicating a mechanism for laminaribiose utilisation. The genome of W. cibaria strain 92 harbours genes for utilising the phosphoketolase pathway for the production of both acetate and lactate from pentose and hexose sugars but lacks two genes necessary for utilising the pentose phosphate pathway. The ability of W. cibaria strain 92 to utilise several types of oligosaccharides derived from dietary fibres, and produce lactate and acetate makes it interesting as a probiotic candidate for further evaluation. Nature Publishing Group UK 2020-04-03 /pmc/articles/PMC7125115/ /pubmed/32246087 http://dx.doi.org/10.1038/s41598-020-62610-x Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Månberger, Anna Verbrugghe, Phebe Guðmundsdóttir, Elísabet Eik Santesson, Sara Nilsson, Anne Hreggviðsson, Guðmundur Óli Linares-Pastén, Javier A. Nordberg Karlsson, Eva Taxogenomic assessment and genomic characterisation of Weissella cibaria strain 92 able to metabolise oligosaccharides derived from dietary fibres |
title | Taxogenomic assessment and genomic characterisation of Weissella cibaria strain 92 able to metabolise oligosaccharides derived from dietary fibres |
title_full | Taxogenomic assessment and genomic characterisation of Weissella cibaria strain 92 able to metabolise oligosaccharides derived from dietary fibres |
title_fullStr | Taxogenomic assessment and genomic characterisation of Weissella cibaria strain 92 able to metabolise oligosaccharides derived from dietary fibres |
title_full_unstemmed | Taxogenomic assessment and genomic characterisation of Weissella cibaria strain 92 able to metabolise oligosaccharides derived from dietary fibres |
title_short | Taxogenomic assessment and genomic characterisation of Weissella cibaria strain 92 able to metabolise oligosaccharides derived from dietary fibres |
title_sort | taxogenomic assessment and genomic characterisation of weissella cibaria strain 92 able to metabolise oligosaccharides derived from dietary fibres |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7125115/ https://www.ncbi.nlm.nih.gov/pubmed/32246087 http://dx.doi.org/10.1038/s41598-020-62610-x |
work_keys_str_mv | AT manbergeranna taxogenomicassessmentandgenomiccharacterisationofweissellacibariastrain92abletometaboliseoligosaccharidesderivedfromdietaryfibres AT verbrugghephebe taxogenomicassessmentandgenomiccharacterisationofweissellacibariastrain92abletometaboliseoligosaccharidesderivedfromdietaryfibres AT guðmundsdottirelisabeteik taxogenomicassessmentandgenomiccharacterisationofweissellacibariastrain92abletometaboliseoligosaccharidesderivedfromdietaryfibres AT santessonsara taxogenomicassessmentandgenomiccharacterisationofweissellacibariastrain92abletometaboliseoligosaccharidesderivedfromdietaryfibres AT nilssonanne taxogenomicassessmentandgenomiccharacterisationofweissellacibariastrain92abletometaboliseoligosaccharidesderivedfromdietaryfibres AT hreggviðssonguðmunduroli taxogenomicassessmentandgenomiccharacterisationofweissellacibariastrain92abletometaboliseoligosaccharidesderivedfromdietaryfibres AT linarespastenjaviera taxogenomicassessmentandgenomiccharacterisationofweissellacibariastrain92abletometaboliseoligosaccharidesderivedfromdietaryfibres AT nordbergkarlssoneva taxogenomicassessmentandgenomiccharacterisationofweissellacibariastrain92abletometaboliseoligosaccharidesderivedfromdietaryfibres |