Frameshift mutations in infectious cDNA clones of Citrus tristeza virus: a strategy to minimize the toxicity of viral sequences to Escherichia coli

The advent of reverse genetics revolutionized the study of positive-stranded RNA viruses that were amenable for cloning as cDNAs into high-copy-number plasmids of Escherichia coli. However, some viruses are inherently refractory to cloning in high-copy-number plasmids due to toxicity of viral sequen...

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Detalles Bibliográficos
Autores principales: Satyanarayana, Tatineni, Gowda, Siddarame, Ayllón, María A, Dawson, William O
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Science (USA). 2003
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7125997/
https://www.ncbi.nlm.nih.gov/pubmed/12954215
http://dx.doi.org/10.1016/S0042-6822(03)00387-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7125997/
https://www.ncbi.nlm.nih.gov/pubmed/12954215
http://dx.doi.org/10.1016/S0042-6822(03)00387-8

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