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A general method for the induction and screening of antisera for cDNA-encoded polypeptides: antibodies specific for a coronavirus putative polymerase-encoding gene

A prokaryotic vector, pGE374, containing the recA and lacZ genes, out-of-frame, was used for the expression of cDNA derived from the putative polymerase-encoding gene of the coronavirus mouse hepatitis virus strain A59 (MHV-A59). The pGE374/viral recombinant vector generates a tripartite bacterial/v...

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Detalles Bibliográficos
Autores principales: Zoltick, Philip W., Leibowitz, Julian L., DeVries, James R., Weinstock, George M., Weiss, Susan R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1989
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7127337/
https://www.ncbi.nlm.nih.gov/pubmed/2560756
http://dx.doi.org/10.1016/0378-1119(89)90434-4