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Attenuation of SARS coronavirus by a short hairpin RNA expression plasmid targeting RNA-dependent RNA polymerase()
Severe acute respiratory syndrome (SARS) is a highly contagious and sometimes a lethal disease, which spread over five continents in 2002–2003. Laboratory analysis showed that the etiologic agent for SARS is a new type of coronavirus. Currently, there is no specific treatment for this disease. RNA i...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Inc.
2004
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7127537/ https://www.ncbi.nlm.nih.gov/pubmed/15183056 http://dx.doi.org/10.1016/j.virol.2004.03.031 |
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author | Lu, Aili Zhang, Huanqing Zhang, Xiaoyan Wang, Hongxia Hu, Qikuan Shen, Li Schaffhausen, Brian S. Hou, Weimin Li, Linsong |
author_facet | Lu, Aili Zhang, Huanqing Zhang, Xiaoyan Wang, Hongxia Hu, Qikuan Shen, Li Schaffhausen, Brian S. Hou, Weimin Li, Linsong |
author_sort | Lu, Aili |
collection | PubMed |
description | Severe acute respiratory syndrome (SARS) is a highly contagious and sometimes a lethal disease, which spread over five continents in 2002–2003. Laboratory analysis showed that the etiologic agent for SARS is a new type of coronavirus. Currently, there is no specific treatment for this disease. RNA interference (RNAi) is a recently discovered antiviral mechanism in plant and animal cells that induces a specific degradation of double-stranded RNA. Here, we provide evidences that RNAi targeting at coronavirus RNA-dependent RNA polymerase (RDRP) using short hairpin RNA (shRNA) expression plasmids can specifically inhibit expression of extraneous coronavirus RDRP in 293 and HeLa cells. Moreover, this construct significantly reduced the plaque formation of SARS coronaviruses in Vero-E6 cells. The data may suggest a new approach for treatment of SARS patients. |
format | Online Article Text |
id | pubmed-7127537 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2004 |
publisher | Elsevier Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-71275372020-04-08 Attenuation of SARS coronavirus by a short hairpin RNA expression plasmid targeting RNA-dependent RNA polymerase() Lu, Aili Zhang, Huanqing Zhang, Xiaoyan Wang, Hongxia Hu, Qikuan Shen, Li Schaffhausen, Brian S. Hou, Weimin Li, Linsong Virology Article Severe acute respiratory syndrome (SARS) is a highly contagious and sometimes a lethal disease, which spread over five continents in 2002–2003. Laboratory analysis showed that the etiologic agent for SARS is a new type of coronavirus. Currently, there is no specific treatment for this disease. RNA interference (RNAi) is a recently discovered antiviral mechanism in plant and animal cells that induces a specific degradation of double-stranded RNA. Here, we provide evidences that RNAi targeting at coronavirus RNA-dependent RNA polymerase (RDRP) using short hairpin RNA (shRNA) expression plasmids can specifically inhibit expression of extraneous coronavirus RDRP in 293 and HeLa cells. Moreover, this construct significantly reduced the plaque formation of SARS coronaviruses in Vero-E6 cells. The data may suggest a new approach for treatment of SARS patients. Elsevier Inc. 2004-06-20 2004-05-04 /pmc/articles/PMC7127537/ /pubmed/15183056 http://dx.doi.org/10.1016/j.virol.2004.03.031 Text en Copyright © 2004 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Lu, Aili Zhang, Huanqing Zhang, Xiaoyan Wang, Hongxia Hu, Qikuan Shen, Li Schaffhausen, Brian S. Hou, Weimin Li, Linsong Attenuation of SARS coronavirus by a short hairpin RNA expression plasmid targeting RNA-dependent RNA polymerase() |
title | Attenuation of SARS coronavirus by a short hairpin RNA expression plasmid targeting RNA-dependent RNA polymerase() |
title_full | Attenuation of SARS coronavirus by a short hairpin RNA expression plasmid targeting RNA-dependent RNA polymerase() |
title_fullStr | Attenuation of SARS coronavirus by a short hairpin RNA expression plasmid targeting RNA-dependent RNA polymerase() |
title_full_unstemmed | Attenuation of SARS coronavirus by a short hairpin RNA expression plasmid targeting RNA-dependent RNA polymerase() |
title_short | Attenuation of SARS coronavirus by a short hairpin RNA expression plasmid targeting RNA-dependent RNA polymerase() |
title_sort | attenuation of sars coronavirus by a short hairpin rna expression plasmid targeting rna-dependent rna polymerase() |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7127537/ https://www.ncbi.nlm.nih.gov/pubmed/15183056 http://dx.doi.org/10.1016/j.virol.2004.03.031 |
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