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Selection for 3′ end triplets for polymerase chain reaction primers

The 3′ end of a primer is a key component of PCR primer design. Many recommendations for the composition and sequence of the 3′ end have been suggested based on theoretical considerations, but have not been verified experimentally. We analyzed 3′ end triplets of PCR primer sequences obtained from re...

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Detalles Bibliográficos
Autores principales: Onodera, Kenji, Melcher, Ulrich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Ltd. 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7127618/
https://www.ncbi.nlm.nih.gov/pubmed/15488375
http://dx.doi.org/10.1016/j.mcp.2004.05.007