Development of a SYBR Green I based real-time RT-PCR assay for detection and quantification of bovine coronavirus

A novel two-step, SYBR Green I based real-time RT-PCR assay was developed for detection and quantification of BCoV using ABI PRISM 7500 sequence detection system. The assay was carried out using two sets of primers designed to amplify highly conserved sequences of the nucleocapsid gene of BCoV and t...

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Detalles Bibliográficos
Autores principales: Amer, Haitham M., Almajhdi, Fahad N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Ltd. 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7127634/
https://www.ncbi.nlm.nih.gov/pubmed/21419844
http://dx.doi.org/10.1016/j.mcp.2011.03.001

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7127634/
https://www.ncbi.nlm.nih.gov/pubmed/21419844
http://dx.doi.org/10.1016/j.mcp.2011.03.001

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