Establishment of a stable CHO cell line with high level expression of recombinant porcine IFN-β

A CHO cell clone (CHO-PoIFN-β) with stable porcine IFN-β expression under control of CMV promoter was selected under G418 pressure. In a 25 cm(2) cell culture flask (5 ml culture medium), the cumulative protein yield of recombinant PoIFN-β reached 2.3 × 10(6) IU/ml. This cells clone maintained stabl...

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Detalles Bibliográficos
Autores principales: Chen, Weiye, Cao, Wenyan, Zhao, Huijun, Hu, Qianqian, Qu, Linmao, Hu, Sen, Ge, Jinying, Wen, Zhiyuan, Wang, Xijun, Li, HaoBo, Huang, Kehe, Bu, Zhigao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier Ltd. 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7128424/
https://www.ncbi.nlm.nih.gov/pubmed/21459017
http://dx.doi.org/10.1016/j.cyto.2010.12.002

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7128424/
https://www.ncbi.nlm.nih.gov/pubmed/21459017
http://dx.doi.org/10.1016/j.cyto.2010.12.002

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