High-level expression of active recombinant ubiquitin carboxyl-terminal hydrolase of Drosophila melanogaster in Pichia pastoris

Ubiquitin carboxyl-terminal hydrolases (UCHs) are implicated in the proteolytic processing of polymeric ubiquitin. The high specificity for the recognition site makes UCHs useful enzymes for in vitro cleavage of ubiquitin fusion proteins. In this work, an active C-terminal His-tagged UCH from Drosop...

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Detalles Bibliográficos
Autores principales: Jin, Feng-liang, Xu, Xiao-xia, Yu, Xiao-qiang, Ren, Shun-xiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier Inc. 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7128749/
https://www.ncbi.nlm.nih.gov/pubmed/18817875
http://dx.doi.org/10.1016/j.pep.2008.08.010

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7128749/
https://www.ncbi.nlm.nih.gov/pubmed/18817875
http://dx.doi.org/10.1016/j.pep.2008.08.010

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