Molecular cloning, expression, and purification of SARS-CoV nsp13

The SARS-nsp13 protein was identified as an mRNA cap1 methyltransferase. In this study, the nsp13 gene was cloned from the SARS-CoV PUMC02 strain viral RNA by RT-PCR, and inserted into the expression plasmid pET30a(+). The recombinant plasmid pET30a(+)-nsp13 was confirmed by restriction enzymes and...

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Detalles Bibliográficos
Autores principales: Fan, Zheng, Peng, Kunpeng, Tan, Xinyu, Yin, Bin, Dong, Xiuhua, Qiu, Feichan, Shen, Yan, Wang, Heng, Yuan, Jiangang, Qiang, Boqin, Peng, Xiaozhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2005
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7129550/
https://www.ncbi.nlm.nih.gov/pubmed/15866708
http://dx.doi.org/10.1016/j.pep.2004.08.003

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7129550/
https://www.ncbi.nlm.nih.gov/pubmed/15866708
http://dx.doi.org/10.1016/j.pep.2004.08.003

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