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A one step quantitative RT-PCR for detection of SARS coronavirus with an internal control for PCR inhibitors

In this study, we report a one step quantitative RT-PCR assay for severe acute respiratory syndrome (SARS) diagnosis. The overall detection rate for clinical samples collected from Days 1 to 9 of disease onset is 86.2% and the specificity of the assay is 100%. To detect false negative results due to...

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Detalles Bibliográficos
Autores principales: Poon, Leo L.M, Wong, Bonnie W.Y, Chan, Kwok Hung, Leung, Cynthia S.W, Yuen, Kwok Yung, Guan, Yi, Peiris, Joseph S.M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7129749/
https://www.ncbi.nlm.nih.gov/pubmed/15135737
http://dx.doi.org/10.1016/j.jcv.2003.12.007