A one step quantitative RT-PCR for detection of SARS coronavirus with an internal control for PCR inhibitors

In this study, we report a one step quantitative RT-PCR assay for severe acute respiratory syndrome (SARS) diagnosis. The overall detection rate for clinical samples collected from Days 1 to 9 of disease onset is 86.2% and the specificity of the assay is 100%. To detect false negative results due to...

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Detalles Bibliográficos
Autores principales: Poon, Leo L.M, Wong, Bonnie W.Y, Chan, Kwok Hung, Leung, Cynthia S.W, Yuen, Kwok Yung, Guan, Yi, Peiris, Joseph S.M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2004
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7129749/
https://www.ncbi.nlm.nih.gov/pubmed/15135737
http://dx.doi.org/10.1016/j.jcv.2003.12.007
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author Poon, Leo L.M
Wong, Bonnie W.Y
Chan, Kwok Hung
Leung, Cynthia S.W
Yuen, Kwok Yung
Guan, Yi
Peiris, Joseph S.M
author_facet Poon, Leo L.M
Wong, Bonnie W.Y
Chan, Kwok Hung
Leung, Cynthia S.W
Yuen, Kwok Yung
Guan, Yi
Peiris, Joseph S.M
author_sort Poon, Leo L.M
collection PubMed
description In this study, we report a one step quantitative RT-PCR assay for severe acute respiratory syndrome (SARS) diagnosis. The overall detection rate for clinical samples collected from Days 1 to 9 of disease onset is 86.2% and the specificity of the assay is 100%. To detect false negative results due to PCR inhibitors or faulty RNA extraction, we have further modified this one step RT-PCR assay for simultaneous detection of severe acute respiratory syndrome (SARS) coronavirus (CoV) and 18S ribosomal RNA (rRNA) as an internal positive control.
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spelling pubmed-71297492020-04-08 A one step quantitative RT-PCR for detection of SARS coronavirus with an internal control for PCR inhibitors Poon, Leo L.M Wong, Bonnie W.Y Chan, Kwok Hung Leung, Cynthia S.W Yuen, Kwok Yung Guan, Yi Peiris, Joseph S.M J Clin Virol Article In this study, we report a one step quantitative RT-PCR assay for severe acute respiratory syndrome (SARS) diagnosis. The overall detection rate for clinical samples collected from Days 1 to 9 of disease onset is 86.2% and the specificity of the assay is 100%. To detect false negative results due to PCR inhibitors or faulty RNA extraction, we have further modified this one step RT-PCR assay for simultaneous detection of severe acute respiratory syndrome (SARS) coronavirus (CoV) and 18S ribosomal RNA (rRNA) as an internal positive control. Elsevier B.V. 2004-07 2004-02-03 /pmc/articles/PMC7129749/ /pubmed/15135737 http://dx.doi.org/10.1016/j.jcv.2003.12.007 Text en Copyright © 2003 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Poon, Leo L.M
Wong, Bonnie W.Y
Chan, Kwok Hung
Leung, Cynthia S.W
Yuen, Kwok Yung
Guan, Yi
Peiris, Joseph S.M
A one step quantitative RT-PCR for detection of SARS coronavirus with an internal control for PCR inhibitors
title A one step quantitative RT-PCR for detection of SARS coronavirus with an internal control for PCR inhibitors
title_full A one step quantitative RT-PCR for detection of SARS coronavirus with an internal control for PCR inhibitors
title_fullStr A one step quantitative RT-PCR for detection of SARS coronavirus with an internal control for PCR inhibitors
title_full_unstemmed A one step quantitative RT-PCR for detection of SARS coronavirus with an internal control for PCR inhibitors
title_short A one step quantitative RT-PCR for detection of SARS coronavirus with an internal control for PCR inhibitors
title_sort one step quantitative rt-pcr for detection of sars coronavirus with an internal control for pcr inhibitors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7129749/
https://www.ncbi.nlm.nih.gov/pubmed/15135737
http://dx.doi.org/10.1016/j.jcv.2003.12.007
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