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Gateway cloning is compatible with protein secretion from Pichia pastoris

Secretion of a recombinant protein from the yeast Pichia pastoris requires the presence of a signal peptide at the amino terminus. Maintaining the full amino acid sequence of the signal peptide is thought to be important for proper signal processing and protein secretion. We show that at least for o...

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Detalles Bibliográficos
Autores principales: Esposito, Dominic, Gillette, William K., Miller, David A., Taylor, Troy E., Frank, Peter H., Hu, Renqui, Bekisz, Joseph, Hernandez, Jessica, Cregg, James M., Zoon, Kathryn C., Hartley, James L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7130006/
https://www.ncbi.nlm.nih.gov/pubmed/15766886
http://dx.doi.org/10.1016/j.pep.2004.12.006