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Isolation of the subunits of the coronavirus envelope glycoprotein E2 by hydroxyapatite high-performance liquid chromatography
The coronavirus glycoprotein E2, which is responsible for virus attachment to cell receptors and virus-induced cell fusion, was purified by solubilization of virions with Triton X-114 and phase fractionation. Native E2 and tryptic subunits of the glycoprotein were separated by size-exclusion high-pe...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Published by Elsevier B.V.
1985
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7130145/ https://www.ncbi.nlm.nih.gov/pubmed/2993328 http://dx.doi.org/10.1016/S0021-9673(01)87445-8 |