Isolation of sequences from a random-sequence expression library that mimic viral epitopes

We describe the use of random peptide sequences for the mapping of antigenic determinants. An oligonucleotide with a completely degenerate sequence of 17 or 23 nucleotides was inserted into a bacterial expression vector. This resulted in an expression library producing random hexa- or octapeptides a...

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Detalles Bibliográficos
Autores principales: Lenstra, Johannes A., Erkens, Joannes H.F., Langeveld, J.G.A., Posthumus, Willem P.A., Meloen, Rob H., Gebauer, Fátima, Correa, Isabela, Enjuanes, Luis, Stanley, Keith K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1992
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131437/
https://www.ncbi.nlm.nih.gov/pubmed/1380046
http://dx.doi.org/10.1016/0022-1759(92)90136-H

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131437/
https://www.ncbi.nlm.nih.gov/pubmed/1380046
http://dx.doi.org/10.1016/0022-1759(92)90136-H

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