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Western and dot immunoblotting analysis of viral antigens and antibodies: Application to murine hepatitis virus
Viral proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred quantitatively to nitrocellulose by electroblotting in SDS-containing buffer. Monoclonal antibodies directed against previously defined epitopes on the viral proteins were used as pr...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Published by Elsevier B.V.
1984
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131576/ https://www.ncbi.nlm.nih.gov/pubmed/6208281 http://dx.doi.org/10.1016/0022-1759(84)90043-7 |