Permissible peptide insertions surrounding the signal peptide-mature protein junction of the ClpG prepilin: CS31A fimbriae of Escherichia coli as carriers of foreign sequences

The clpG gene, expressing the Escherichia coli major CS31A fimbrial subunit ClpG, was subjected to random mutagenesis by insertion of an EcoRI linker and a kanamycin-resistance (Km(R)) cassette into the multiple newly generated EcoRI sites. The Km(R) gene was then excised by PstI, which left a 48-bp...

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Detalles Bibliográficos
Autores principales: Der Vartanian, Maurice, Méchin, Marie-Claire, Jaffeux, Bernard, Bertin, Yolande, Félix, Isabelle, Gaillard-Martinie, Brigitte
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1994
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131889/
https://www.ncbi.nlm.nih.gov/pubmed/7523252
http://dx.doi.org/10.1016/0378-1119(94)90229-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131889/
https://www.ncbi.nlm.nih.gov/pubmed/7523252
http://dx.doi.org/10.1016/0378-1119(94)90229-1

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