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Permissible peptide insertions surrounding the signal peptide-mature protein junction of the ClpG prepilin: CS31A fimbriae of Escherichia coli as carriers of foreign sequences
The clpG gene, expressing the Escherichia coli major CS31A fimbrial subunit ClpG, was subjected to random mutagenesis by insertion of an EcoRI linker and a kanamycin-resistance (Km(R)) cassette into the multiple newly generated EcoRI sites. The Km(R) gene was then excised by PstI, which left a 48-bp...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Published by Elsevier B.V.
1994
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7131889/ https://www.ncbi.nlm.nih.gov/pubmed/7523252 http://dx.doi.org/10.1016/0378-1119(94)90229-1 |