In vivo and in vitro models of demyelinating disease X. A Schwannoma-L-2 somatic cell hybrid persistently yielding high titres of mouse hepatitis virus strain JHM

Following infection of RN2 rat Schwannoma cells with unfiltered JHMV inocula, a cell line with an altered phenotype evolved, which was shown to be a somatic cell hybrid of RN2 and mouse L-2 cells. This cell line, EJ, persistently yields JHMV at titres greater than 10(6) pfu/ml and does not show the...

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Autores principales: Coulter-Mackie, M.B., Flintoff, W.F., Dales, S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 1984
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7134070/
https://www.ncbi.nlm.nih.gov/pubmed/6099665
http://dx.doi.org/10.1016/0168-1702(84)90005-4
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author Coulter-Mackie, M.B.
Flintoff, W.F.
Dales, S.
author_facet Coulter-Mackie, M.B.
Flintoff, W.F.
Dales, S.
author_sort Coulter-Mackie, M.B.
collection PubMed
description Following infection of RN2 rat Schwannoma cells with unfiltered JHMV inocula, a cell line with an altered phenotype evolved, which was shown to be a somatic cell hybrid of RN2 and mouse L-2 cells. This cell line, EJ, persistently yields JHMV at titres greater than 10(6) pfu/ml and does not show the suppression of virus production at 39.5 °C that is characteristic of a persistently infected RN2 line. Intracellular viral nucleocapsids are demonstrated. Cloning of EJ hybrids yields cell lines that show a variety of responses to infection by JHMV or MHV(3).
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spelling pubmed-71340702020-04-08 In vivo and in vitro models of demyelinating disease X. A Schwannoma-L-2 somatic cell hybrid persistently yielding high titres of mouse hepatitis virus strain JHM Coulter-Mackie, M.B. Flintoff, W.F. Dales, S. Virus Res Article Following infection of RN2 rat Schwannoma cells with unfiltered JHMV inocula, a cell line with an altered phenotype evolved, which was shown to be a somatic cell hybrid of RN2 and mouse L-2 cells. This cell line, EJ, persistently yields JHMV at titres greater than 10(6) pfu/ml and does not show the suppression of virus production at 39.5 °C that is characteristic of a persistently infected RN2 line. Intracellular viral nucleocapsids are demonstrated. Cloning of EJ hybrids yields cell lines that show a variety of responses to infection by JHMV or MHV(3). Published by Elsevier B.V. 1984-09 2002-11-12 /pmc/articles/PMC7134070/ /pubmed/6099665 http://dx.doi.org/10.1016/0168-1702(84)90005-4 Text en Copyright © 1984 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Coulter-Mackie, M.B.
Flintoff, W.F.
Dales, S.
In vivo and in vitro models of demyelinating disease X. A Schwannoma-L-2 somatic cell hybrid persistently yielding high titres of mouse hepatitis virus strain JHM
title In vivo and in vitro models of demyelinating disease X. A Schwannoma-L-2 somatic cell hybrid persistently yielding high titres of mouse hepatitis virus strain JHM
title_full In vivo and in vitro models of demyelinating disease X. A Schwannoma-L-2 somatic cell hybrid persistently yielding high titres of mouse hepatitis virus strain JHM
title_fullStr In vivo and in vitro models of demyelinating disease X. A Schwannoma-L-2 somatic cell hybrid persistently yielding high titres of mouse hepatitis virus strain JHM
title_full_unstemmed In vivo and in vitro models of demyelinating disease X. A Schwannoma-L-2 somatic cell hybrid persistently yielding high titres of mouse hepatitis virus strain JHM
title_short In vivo and in vitro models of demyelinating disease X. A Schwannoma-L-2 somatic cell hybrid persistently yielding high titres of mouse hepatitis virus strain JHM
title_sort in vivo and in vitro models of demyelinating disease x. a schwannoma-l-2 somatic cell hybrid persistently yielding high titres of mouse hepatitis virus strain jhm
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7134070/
https://www.ncbi.nlm.nih.gov/pubmed/6099665
http://dx.doi.org/10.1016/0168-1702(84)90005-4
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