Characterization of SARS‐CoV main protease and identification of biologically active small molecule inhibitors using a continuous fluorescence‐based assay

Severe acute respiratory syndrome associated coronavirus main protease (SARS‐CoV M(pro)) has been proposed as a prime target for anti‐SARS drug development. We have cloned and overexpressed the SARS‐CoV M(pro) in Escherichia coli, and purified the recombinant M(pro) to homogeneity. The kinetic param...

Descripción completa

Detalles Bibliográficos
Autores principales: Kao, Richard Y., To, Amanda P.C., Ng, Louisa W.Y., Tsui, Wayne H.W., Lee, Terri S.W., Tsoi, Hoi-Wah, Yuen, Kwok-Yung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2004
Materias:
Short Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7134596/
https://www.ncbi.nlm.nih.gov/pubmed/15498556
http://dx.doi.org/10.1016/j.febslet.2004.09.026

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7134596/
https://www.ncbi.nlm.nih.gov/pubmed/15498556
http://dx.doi.org/10.1016/j.febslet.2004.09.026

Ejemplares similares