Characterization of SARS‐CoV main protease and identification of biologically active small molecule inhibitors using a continuous fluorescence‐based assay

Severe acute respiratory syndrome associated coronavirus main protease (SARS‐CoV M(pro)) has been proposed as a prime target for anti‐SARS drug development. We have cloned and overexpressed the SARS‐CoV M(pro) in Escherichia coli, and purified the recombinant M(pro) to homogeneity. The kinetic param...

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Detalles Bibliográficos
Autores principales: Kao, Richard Y., To, Amanda P.C., Ng, Louisa W.Y., Tsui, Wayne H.W., Lee, Terri S.W., Tsoi, Hoi-Wah, Yuen, Kwok-Yung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2004
Materias:
Short Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7134596/
https://www.ncbi.nlm.nih.gov/pubmed/15498556
http://dx.doi.org/10.1016/j.febslet.2004.09.026

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