Cloning and Expression of a Novel Leucine Dehydrogenase: Characterization and L-tert-Leucine Production

Among many genes encoding for amino acid dehydrogenase, a novel leucine dehydrogenase gene from Exiguobacterium sibiricum (EsiLeuDH) was isolated by using genome mining strategy. EsiLeuDH was overexpressed in Escherichia coli BL21 (DE3), followed by purification and characterization. The high thermo...

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Detalles Bibliográficos
Autores principales: Luo, Wei, Zhu, Jing, Zhao, Yuzheng, Zhang, Huili, Yang, Xue, Liu, Yuantao, Rao, Zhiming, Yu, Xiaobin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Bioengineering and Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7136578/
https://www.ncbi.nlm.nih.gov/pubmed/32296684
http://dx.doi.org/10.3389/fbioe.2020.00186

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7136578/
https://www.ncbi.nlm.nih.gov/pubmed/32296684
http://dx.doi.org/10.3389/fbioe.2020.00186

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