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Development of a system for efficient callus production, somatic embryogenesis and gene editing using CRISPR/Cas9 in Saffron (Crocus sativus L.)

BACKGROUND: Crocus sativus is a recalcitrant plant for genetic transformation and genetic improvement, largely due to difficulties in Agrobacterium mediated transformation and vegetative reproduction. Effective genome editing requires proficient callus production and an efficient method to deliver C...

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Detalles Bibliográficos
Autores principales: Chib, Sudha, Thangaraj, Arulprakash, Kaul, Sanjana, Dhar, Manoj Kumar, Kaul, Tanushri
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137501/
https://www.ncbi.nlm.nih.gov/pubmed/32280363
http://dx.doi.org/10.1186/s13007-020-00589-2