A 6-nm ultra-photostable DNA FluoroCube for fluorescence imaging

Photobleaching limits extended imaging of fluorescent biological samples. Here, we developed DNA based “FluoroCubes” that are similar in size to the green fluorescent protein (GFP), have single-point attachment to proteins, have a ~54-fold higher photobleaching lifetime and emit ~43-fold more photon...

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Detalles Bibliográficos
Autores principales: Niekamp, Stefan, Stuurman, Nico, Vale, Ronald D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7138518/
https://www.ncbi.nlm.nih.gov/pubmed/32203385
http://dx.doi.org/10.1038/s41592-020-0782-3
Descripción
Sumario:Photobleaching limits extended imaging of fluorescent biological samples. Here, we developed DNA based “FluoroCubes” that are similar in size to the green fluorescent protein (GFP), have single-point attachment to proteins, have a ~54-fold higher photobleaching lifetime and emit ~43-fold more photons than single organic dyes. We demonstrate that DNA FluoroCubes provide outstanding tools for single-molecule imaging, allowing the tracking of single motor proteins for >800 steps with nanometer precision.

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