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A 6-nm ultra-photostable DNA FluoroCube for fluorescence imaging
Photobleaching limits extended imaging of fluorescent biological samples. Here, we developed DNA based “FluoroCubes” that are similar in size to the green fluorescent protein (GFP), have single-point attachment to proteins, have a ~54-fold higher photobleaching lifetime and emit ~43-fold more photon...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7138518/ https://www.ncbi.nlm.nih.gov/pubmed/32203385 http://dx.doi.org/10.1038/s41592-020-0782-3 |
| _version_ | 1783518585670336512 |
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| author | Niekamp, Stefan Stuurman, Nico Vale, Ronald D. |
| author_facet | Niekamp, Stefan Stuurman, Nico Vale, Ronald D. |
| author_sort | Niekamp, Stefan |
| collection | PubMed |
| description | Photobleaching limits extended imaging of fluorescent biological samples. Here, we developed DNA based “FluoroCubes” that are similar in size to the green fluorescent protein (GFP), have single-point attachment to proteins, have a ~54-fold higher photobleaching lifetime and emit ~43-fold more photons than single organic dyes. We demonstrate that DNA FluoroCubes provide outstanding tools for single-molecule imaging, allowing the tracking of single motor proteins for >800 steps with nanometer precision. |
| format | Online Article Text |
| id | pubmed-7138518 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2020 |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-71385182020-09-16 A 6-nm ultra-photostable DNA FluoroCube for fluorescence imaging Niekamp, Stefan Stuurman, Nico Vale, Ronald D. Nat Methods Article Photobleaching limits extended imaging of fluorescent biological samples. Here, we developed DNA based “FluoroCubes” that are similar in size to the green fluorescent protein (GFP), have single-point attachment to proteins, have a ~54-fold higher photobleaching lifetime and emit ~43-fold more photons than single organic dyes. We demonstrate that DNA FluoroCubes provide outstanding tools for single-molecule imaging, allowing the tracking of single motor proteins for >800 steps with nanometer precision. 2020-03-16 2020-04 /pmc/articles/PMC7138518/ /pubmed/32203385 http://dx.doi.org/10.1038/s41592-020-0782-3 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
| spellingShingle | Article Niekamp, Stefan Stuurman, Nico Vale, Ronald D. A 6-nm ultra-photostable DNA FluoroCube for fluorescence imaging |
| title | A 6-nm ultra-photostable DNA FluoroCube for fluorescence imaging |
| title_full | A 6-nm ultra-photostable DNA FluoroCube for fluorescence imaging |
| title_fullStr | A 6-nm ultra-photostable DNA FluoroCube for fluorescence imaging |
| title_full_unstemmed | A 6-nm ultra-photostable DNA FluoroCube for fluorescence imaging |
| title_short | A 6-nm ultra-photostable DNA FluoroCube for fluorescence imaging |
| title_sort | 6-nm ultra-photostable dna fluorocube for fluorescence imaging |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7138518/ https://www.ncbi.nlm.nih.gov/pubmed/32203385 http://dx.doi.org/10.1038/s41592-020-0782-3 |
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