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Validation of Reference Genes for Gene Expression Studies by RT-qPCR in HepaRG Cells during Toxicity Testing and Disease Modelling

Gene expression analysis by quantitative real-time polymerase chain reaction (RT-qPCR) is routinely used in biomedical studies. The reproducibility and reliability of the data fundamentally depends on experimental design and data interpretation. Despite the wide application of this assay, there is s...

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Detalles Bibliográficos
Autores principales: Brzeszczyńska, Joanna, Brzeszczyński, Filip, Samuel, Kay, Morgan, Katie, Morley, Steven D., Plevris, John N., Hayes, Peter C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7140694/
https://www.ncbi.nlm.nih.gov/pubmed/32245194
http://dx.doi.org/10.3390/cells9030770