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Cooperativity of K(v)7.4 channels confers ultrafast electromechanical sensitivity and emergent properties in cochlear outer hair cells

The mammalian cochlea relies on active electromotility of outer hair cells (OHCs) to resolve sound frequencies. OHCs use ionic channels and somatic electromotility to achieve the process. It is unclear, though, how the kinetics of voltage-gated ionic channels operate to overcome extrinsic viscous dr...

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Autores principales: Perez-Flores, Maria C., Lee, Jeong H., Park, Seojin, Zhang, Xiao-Dong, Sihn, Choong-Ryoul, Ledford, Hannah A., Wang, Wenying, Kim, Hyo Jeong, Timofeyev, Valeriy, Yarov-Yarovoy, Vladimir, Chiamvimonvat, Nipavan, Rabbitt, Richard D., Yamoah, Ebenezer N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7141818/
https://www.ncbi.nlm.nih.gov/pubmed/32285007
http://dx.doi.org/10.1126/sciadv.aba1104
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author Perez-Flores, Maria C.
Lee, Jeong H.
Park, Seojin
Zhang, Xiao-Dong
Sihn, Choong-Ryoul
Ledford, Hannah A.
Wang, Wenying
Kim, Hyo Jeong
Timofeyev, Valeriy
Yarov-Yarovoy, Vladimir
Chiamvimonvat, Nipavan
Rabbitt, Richard D.
Yamoah, Ebenezer N.
author_facet Perez-Flores, Maria C.
Lee, Jeong H.
Park, Seojin
Zhang, Xiao-Dong
Sihn, Choong-Ryoul
Ledford, Hannah A.
Wang, Wenying
Kim, Hyo Jeong
Timofeyev, Valeriy
Yarov-Yarovoy, Vladimir
Chiamvimonvat, Nipavan
Rabbitt, Richard D.
Yamoah, Ebenezer N.
author_sort Perez-Flores, Maria C.
collection PubMed
description The mammalian cochlea relies on active electromotility of outer hair cells (OHCs) to resolve sound frequencies. OHCs use ionic channels and somatic electromotility to achieve the process. It is unclear, though, how the kinetics of voltage-gated ionic channels operate to overcome extrinsic viscous drag on OHCs at high frequency. Here, we report ultrafast electromechanical gating of clustered K(v)7.4 in OHCs. Increases in kinetics and sensitivity resulting from cooperativity among clustered-K(v)7.4 were revealed, using optogenetics strategies. Upon clustering, the half-activation voltage shifted negative, and the speed of activation increased relative to solitary channels. Clustering also rendered K(v)7.4 channels mechanically sensitive, confirmed in consolidated K(v)7.4 channels at the base of OHCs. K(v)7.4 clusters provide OHCs with ultrafast electromechanical channel gating, varying in magnitude and speed along the cochlea axis. Ultrafast K(v)7.4 gating provides OHCs with a feedback mechanism that enables the cochlea to overcome viscous drag and resolve sounds at auditory frequencies.
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spelling pubmed-71418182020-04-13 Cooperativity of K(v)7.4 channels confers ultrafast electromechanical sensitivity and emergent properties in cochlear outer hair cells Perez-Flores, Maria C. Lee, Jeong H. Park, Seojin Zhang, Xiao-Dong Sihn, Choong-Ryoul Ledford, Hannah A. Wang, Wenying Kim, Hyo Jeong Timofeyev, Valeriy Yarov-Yarovoy, Vladimir Chiamvimonvat, Nipavan Rabbitt, Richard D. Yamoah, Ebenezer N. Sci Adv Research Articles The mammalian cochlea relies on active electromotility of outer hair cells (OHCs) to resolve sound frequencies. OHCs use ionic channels and somatic electromotility to achieve the process. It is unclear, though, how the kinetics of voltage-gated ionic channels operate to overcome extrinsic viscous drag on OHCs at high frequency. Here, we report ultrafast electromechanical gating of clustered K(v)7.4 in OHCs. Increases in kinetics and sensitivity resulting from cooperativity among clustered-K(v)7.4 were revealed, using optogenetics strategies. Upon clustering, the half-activation voltage shifted negative, and the speed of activation increased relative to solitary channels. Clustering also rendered K(v)7.4 channels mechanically sensitive, confirmed in consolidated K(v)7.4 channels at the base of OHCs. K(v)7.4 clusters provide OHCs with ultrafast electromechanical channel gating, varying in magnitude and speed along the cochlea axis. Ultrafast K(v)7.4 gating provides OHCs with a feedback mechanism that enables the cochlea to overcome viscous drag and resolve sounds at auditory frequencies. American Association for the Advancement of Science 2020-04-08 /pmc/articles/PMC7141818/ /pubmed/32285007 http://dx.doi.org/10.1126/sciadv.aba1104 Text en Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (http://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
spellingShingle Research Articles
Perez-Flores, Maria C.
Lee, Jeong H.
Park, Seojin
Zhang, Xiao-Dong
Sihn, Choong-Ryoul
Ledford, Hannah A.
Wang, Wenying
Kim, Hyo Jeong
Timofeyev, Valeriy
Yarov-Yarovoy, Vladimir
Chiamvimonvat, Nipavan
Rabbitt, Richard D.
Yamoah, Ebenezer N.
Cooperativity of K(v)7.4 channels confers ultrafast electromechanical sensitivity and emergent properties in cochlear outer hair cells
title Cooperativity of K(v)7.4 channels confers ultrafast electromechanical sensitivity and emergent properties in cochlear outer hair cells
title_full Cooperativity of K(v)7.4 channels confers ultrafast electromechanical sensitivity and emergent properties in cochlear outer hair cells
title_fullStr Cooperativity of K(v)7.4 channels confers ultrafast electromechanical sensitivity and emergent properties in cochlear outer hair cells
title_full_unstemmed Cooperativity of K(v)7.4 channels confers ultrafast electromechanical sensitivity and emergent properties in cochlear outer hair cells
title_short Cooperativity of K(v)7.4 channels confers ultrafast electromechanical sensitivity and emergent properties in cochlear outer hair cells
title_sort cooperativity of k(v)7.4 channels confers ultrafast electromechanical sensitivity and emergent properties in cochlear outer hair cells
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7141818/
https://www.ncbi.nlm.nih.gov/pubmed/32285007
http://dx.doi.org/10.1126/sciadv.aba1104
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