Complementing 16S rRNA Gene Amplicon Sequencing with Total Bacterial Load To Infer Absolute Species Concentrations in the Vaginal Microbiome

Whereas 16S rRNA gene amplicon sequencing quantifies relative abundances of bacterial taxa, variation in total bacterial load between samples restricts its ability to reflect absolute concentrations of individual bacterial species. Quantitative PCR (qPCR) can quantify individual species, but it is n...

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Detalles Bibliográficos
Autores principales: Tettamanti Boshier, Florencia A., Srinivasan, Sujatha, Lopez, Anthony, Hoffman, Noah G., Proll, Sean, Fredricks, David N., Schiffer, Joshua T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2020
Materias:
Methods and Protocols
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7141891/
https://www.ncbi.nlm.nih.gov/pubmed/32265316
http://dx.doi.org/10.1128/mSystems.00777-19

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7141891/
https://www.ncbi.nlm.nih.gov/pubmed/32265316
http://dx.doi.org/10.1128/mSystems.00777-19

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