Cargando…

Complementing 16S rRNA Gene Amplicon Sequencing with Total Bacterial Load To Infer Absolute Species Concentrations in the Vaginal Microbiome

Whereas 16S rRNA gene amplicon sequencing quantifies relative abundances of bacterial taxa, variation in total bacterial load between samples restricts its ability to reflect absolute concentrations of individual bacterial species. Quantitative PCR (qPCR) can quantify individual species, but it is n...

Descripción completa

Detalles Bibliográficos
Autores principales:	Tettamanti Boshier, Florencia A., Srinivasan, Sujatha, Lopez, Anthony, Hoffman, Noah G., Proll, Sean, Fredricks, David N., Schiffer, Joshua T.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society for Microbiology 2020
Materias:	Methods and Protocols
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7141891/ https://www.ncbi.nlm.nih.gov/pubmed/32265316 http://dx.doi.org/10.1128/mSystems.00777-19

Ejemplares similares

Optimizing metaproteomics database construction: lessons from a study of the vaginal microbiome
por: Lee, Elliot M., et al.
Publicado: (2023)

High-Throughput Miniaturized 16S rRNA Amplicon Library Preparation Reduces Costs while Preserving Microbiome Integrity
por: Minich, Jeremiah J., et al.
Publicado: (2018)

Evaluating the accuracy of amplicon-based microbiome computational pipelines on simulated human gut microbial communities
por: Golob, Jonathan L., et al.
Publicado: (2017)

Genital Herpes Simplex Virus Type 2 Suppression With Valacyclovir Is Not Associated With Changes in Nugent Score or Absolute Abundance of Key Vaginal Bacteria
por: Babu, Tara M, et al.
Publicado: (2023)

The Human Vaginal Bacterial Biota and Bacterial Vaginosis
por: Srinivasan, Sujatha, et al.
Publicado: (2008)

Optimization of high-throughput 16S rRNA gene amplicon sequencing: an assessment of PCR pooling, mastermix use and contamination
por: Aggarwal, Dinesh, et al.
Publicado: (2023)

Synthetic spike-in standards for high-throughput 16S rRNA gene amplicon sequencing
por: Tourlousse, Dieter M., et al.
Publicado: (2017)

Vaginal and Extra-Vaginal Bacterial Colonization and Risk for Incident Bacterial Vaginosis in a Population of Women Who Have Sex With Men
por: Fredricks, David N, et al.
Publicado: (2020)

RiboFR-Seq: a novel approach to linking 16S rRNA amplicon profiles to metagenomes
por: Zhang, Yanming, et al.
Publicado: (2016)

High-throughput amplicon sequencing of the full-length 16S rRNA gene with single-nucleotide resolution
por: Callahan, Benjamin J, et al.
Publicado: (2019)

A tool kit for quantifying eukaryotic rRNA gene sequences from human microbiome samples
por: Dollive, Serena, et al.
Publicado: (2012)

High-resolution microbiome analysis enabled by linking of 16S rRNA gene sequences with adjacent genomic contexts
por: Kapustina, Žana, et al.
Publicado: (2021)

A georeferenced rRNA amplicon database of aquatic microbiomes from South America
por: Metz, Sebastian, et al.
Publicado: (2022)

More Than Meets the Eye: Associations of Vaginal Bacteria with Gram Stain Morphotypes Using Molecular Phylogenetic Analysis
por: Srinivasan, Sujatha, et al.
Publicado: (2013)

MDSINE: Microbial Dynamical Systems INference Engine for microbiome time-series analyses
por: Bucci, Vanni, et al.
Publicado: (2016)

Benchmarking Bioinformatic Tools for Amplicon-Based Sequencing of Norovirus
por: Fitzpatrick, Amy H., et al.
Publicado: (2022)

Analysis of the mouse gut microbiome using full-length 16S rRNA amplicon sequencing
por: Shin, Jongoh, et al.
Publicado: (2016)

MetaSquare: an integrated metadatabase of 16S rRNA gene amplicon for microbiome taxonomic classification
por: Liao, Chun-Chieh, et al.
Publicado: (2022)

Long-read amplicon denoising
por: Kumar, Venkatesh, et al.
Publicado: (2019)

Associations Between Vaginal Bacteria and Bacterial Vaginosis Signs and Symptoms: A Comparative Study of Kenyan and American Women
por: Carter, Kayla A., et al.
Publicado: (2022)

MinION™ Nanopore Sequencing of Skin Microbiome 16S and 16S-23S rRNA Gene Amplicons
por: Rozas, Miquel, et al.
Publicado: (2022)

Correction to: MetaSquare: an integrated metadatabase of 16S rRNA gene amplicon for microbiome taxonomic classification
Publicado: (2022)

Reproductive Microbiomes in Domestic Livestock: Insights Utilizing 16S rRNA Gene Amplicon Community Sequencing
por: Poole, Rebecca K., et al.
Publicado: (2023)

Best practices for generating and analyzing 16S rRNA amplicon data to track coral microbiome dynamics
por: Silva, Denise P., et al.
Publicado: (2023)

Comprehensive Assessment of 16S rRNA Gene Amplicon Sequencing for Microbiome Profiling across Multiple Habitats
por: Zhang, Wenke, et al.
Publicado: (2023)

Association between vaginal washing and vaginal bacterial concentrations
por: Sabo, Michelle C., et al.
Publicado: (2019)

Evaluation of Hybridization Capture Versus Amplicon‐Based Methods for Whole‐Exome Sequencing
por: Samorodnitsky, Eric, et al.
Publicado: (2015)

DEN-IM: dengue virus genotyping from amplicon and shotgun metagenomic sequencing
por: Mendes, Catarina I., et al.
Publicado: (2020)

Amplicon-Based High-Throughput Sequencing Method for Genotypic Characterization of Norovirus in Oysters
por: Fitzpatrick, Amy H., et al.
Publicado: (2023)

Absolute nucleosome occupancy map for the Saccharomyces cerevisiae genome
por: Oberbeckmann, Elisa, et al.
Publicado: (2019)

Comparison of paperpoint and curette sampling of subgingival microbiome composition as analyzed by 16S rRNA gene amplicon sequencing
por: Beyer, K., et al.
Publicado: (2017)

Gut Microbiome 16S rRNA Gene Amplicon Taxonomic Profiling of Hospitalized Moroccan COVID-19 Patients
por: Sehli, Sofia, et al.
Publicado: (2022)

Oncogene Concatenated Enriched Amplicon Nanopore Sequencing for rapid, accurate, and affordable somatic mutation detection
por: Thirunavukarasu, Deepak, et al.
Publicado: (2021)

Comparison of iSeq and MiSeq as the two platforms for 16S rRNA sequencing in the study of the gut of rat microbiome
por: Salamon, Dominika, et al.
Publicado: (2022)

Grinder: a versatile amplicon and shotgun sequence simulator
por: Angly, Florent E., et al.
Publicado: (2012)

Microbiome Shift, Diversity, and Overabundance of Opportunistic Pathogens in Bovine Digital Dermatitis Revealed by 16S rRNA Amplicon Sequencing
por: Espiritu, Hector M., et al.
Publicado: (2020)

High-Resolution Differentiation of Enteric Bacteria in Premature Infant Fecal Microbiomes Using a Novel rRNA Amplicon
por: Graf, J., et al.
Publicado: (2021)

Curated and harmonized gut microbiome 16S rRNA amplicon data from dietary fiber intervention studies in humans
por: Rodriguez, Cynthia I., et al.
Publicado: (2023)

OTTER, a new method for quantifying absolute amounts of tRNAs
por: Nagai, Akihisa, et al.
Publicado: (2021)

Species-Level Resolution of Female Bladder Microbiota from 16S rRNA Amplicon Sequencing
por: Hoffman, Carter, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_1nr8bd2gmdpf9mid2updh8m97q