Cargando…

Protective Effect of Low-Molecular-Weight Fucoidan on Radiation-Induced Fibrosis Through TGF-β1/Smad Pathway-Mediated Inhibition of Collagen I Accumulation

Radiation-induced fibrosis (RIF) occurs after radiation therapy in normal tissues due to excessive production and deposition of extracellular matrix proteins and collagen, possibly resulting in organ function impairment. This study investigates the effects of low-molecular-weight fucoidan (LMF) on i...

Descripción completa

Detalles Bibliográficos
Autores principales: Wu, Szu-Yuan, Chen, Yu-Ting, Tsai, Guo-Yu, Hsu, Fu-Yin, Hwang, Pai-An
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7142431/
https://www.ncbi.nlm.nih.gov/pubmed/32120789
http://dx.doi.org/10.3390/md18030136
_version_ 1783519379817758720
author Wu, Szu-Yuan
Chen, Yu-Ting
Tsai, Guo-Yu
Hsu, Fu-Yin
Hwang, Pai-An
author_facet Wu, Szu-Yuan
Chen, Yu-Ting
Tsai, Guo-Yu
Hsu, Fu-Yin
Hwang, Pai-An
author_sort Wu, Szu-Yuan
collection PubMed
description Radiation-induced fibrosis (RIF) occurs after radiation therapy in normal tissues due to excessive production and deposition of extracellular matrix proteins and collagen, possibly resulting in organ function impairment. This study investigates the effects of low-molecular-weight fucoidan (LMF) on irradiated NIH3T3 cells. Specifically, we quantified cellular metabolic activity, fibrosis-related mRNA expression, transforming growth factor beta-1 (TGF-β1), and collagen-1 protein expression, and fibroblast contractility in response to LMF. LMF pre + post-treatment could more effectively increase cellular metabolic activity compared with LMF post-treatment. LMF pre + post-treatment inhibited TGF-β1 expression, which mediates negative activation of phosphorylated Smad3 (pSmad3) and Smad4 complex formation and suppresses downstream collagen I accumulation. In addition, LMF pre + post-treatment significantly reduced actin-stress fibers in irradiated NIH3T3 cells. LMF, a natural substance obtained from brown seaweed, may be a candidate agent for preventing or inhibiting RIF.
format Online
Article
Text
id pubmed-7142431
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-71424312020-04-15 Protective Effect of Low-Molecular-Weight Fucoidan on Radiation-Induced Fibrosis Through TGF-β1/Smad Pathway-Mediated Inhibition of Collagen I Accumulation Wu, Szu-Yuan Chen, Yu-Ting Tsai, Guo-Yu Hsu, Fu-Yin Hwang, Pai-An Mar Drugs Article Radiation-induced fibrosis (RIF) occurs after radiation therapy in normal tissues due to excessive production and deposition of extracellular matrix proteins and collagen, possibly resulting in organ function impairment. This study investigates the effects of low-molecular-weight fucoidan (LMF) on irradiated NIH3T3 cells. Specifically, we quantified cellular metabolic activity, fibrosis-related mRNA expression, transforming growth factor beta-1 (TGF-β1), and collagen-1 protein expression, and fibroblast contractility in response to LMF. LMF pre + post-treatment could more effectively increase cellular metabolic activity compared with LMF post-treatment. LMF pre + post-treatment inhibited TGF-β1 expression, which mediates negative activation of phosphorylated Smad3 (pSmad3) and Smad4 complex formation and suppresses downstream collagen I accumulation. In addition, LMF pre + post-treatment significantly reduced actin-stress fibers in irradiated NIH3T3 cells. LMF, a natural substance obtained from brown seaweed, may be a candidate agent for preventing or inhibiting RIF. MDPI 2020-02-27 /pmc/articles/PMC7142431/ /pubmed/32120789 http://dx.doi.org/10.3390/md18030136 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wu, Szu-Yuan
Chen, Yu-Ting
Tsai, Guo-Yu
Hsu, Fu-Yin
Hwang, Pai-An
Protective Effect of Low-Molecular-Weight Fucoidan on Radiation-Induced Fibrosis Through TGF-β1/Smad Pathway-Mediated Inhibition of Collagen I Accumulation
title Protective Effect of Low-Molecular-Weight Fucoidan on Radiation-Induced Fibrosis Through TGF-β1/Smad Pathway-Mediated Inhibition of Collagen I Accumulation
title_full Protective Effect of Low-Molecular-Weight Fucoidan on Radiation-Induced Fibrosis Through TGF-β1/Smad Pathway-Mediated Inhibition of Collagen I Accumulation
title_fullStr Protective Effect of Low-Molecular-Weight Fucoidan on Radiation-Induced Fibrosis Through TGF-β1/Smad Pathway-Mediated Inhibition of Collagen I Accumulation
title_full_unstemmed Protective Effect of Low-Molecular-Weight Fucoidan on Radiation-Induced Fibrosis Through TGF-β1/Smad Pathway-Mediated Inhibition of Collagen I Accumulation
title_short Protective Effect of Low-Molecular-Weight Fucoidan on Radiation-Induced Fibrosis Through TGF-β1/Smad Pathway-Mediated Inhibition of Collagen I Accumulation
title_sort protective effect of low-molecular-weight fucoidan on radiation-induced fibrosis through tgf-β1/smad pathway-mediated inhibition of collagen i accumulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7142431/
https://www.ncbi.nlm.nih.gov/pubmed/32120789
http://dx.doi.org/10.3390/md18030136
work_keys_str_mv AT wuszuyuan protectiveeffectoflowmolecularweightfucoidanonradiationinducedfibrosisthroughtgfb1smadpathwaymediatedinhibitionofcollageniaccumulation
AT chenyuting protectiveeffectoflowmolecularweightfucoidanonradiationinducedfibrosisthroughtgfb1smadpathwaymediatedinhibitionofcollageniaccumulation
AT tsaiguoyu protectiveeffectoflowmolecularweightfucoidanonradiationinducedfibrosisthroughtgfb1smadpathwaymediatedinhibitionofcollageniaccumulation
AT hsufuyin protectiveeffectoflowmolecularweightfucoidanonradiationinducedfibrosisthroughtgfb1smadpathwaymediatedinhibitionofcollageniaccumulation
AT hwangpaian protectiveeffectoflowmolecularweightfucoidanonradiationinducedfibrosisthroughtgfb1smadpathwaymediatedinhibitionofcollageniaccumulation