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Immunoreactivity of Lupine and Soybean Allergens in Foods as Affected by Thermal Processing

Lupine and soybean are important technological aids for the food industry. However, they are also capable of inducing severe allergic reactions in food-sensitized/allergic individuals. In this context, this work intended to study the combined effects of thermal processing and food matrix on the immu...

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Autores principales: Villa, Caterina, Moura, Mónica B. M. V., Costa, Joana, Mafra, Isabel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7142587/
https://www.ncbi.nlm.nih.gov/pubmed/32120788
http://dx.doi.org/10.3390/foods9030254
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author Villa, Caterina
Moura, Mónica B. M. V.
Costa, Joana
Mafra, Isabel
author_facet Villa, Caterina
Moura, Mónica B. M. V.
Costa, Joana
Mafra, Isabel
author_sort Villa, Caterina
collection PubMed
description Lupine and soybean are important technological aids for the food industry. However, they are also capable of inducing severe allergic reactions in food-sensitized/allergic individuals. In this context, this work intended to study the combined effects of thermal processing and food matrix on the immunoreactivity of lupine and soybean proteins used as ingredients in bakery and meat products, respectively. For this purpose, the effects of baking, mild oven cooking, and autoclaving on the protein profiles were evaluated, using model mixtures simulating the production of lupine-containing breads and soybean-containing cooked hams/sausages, by native- and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and immunoblotting using specific antibodies. The results showed that lupine gamma-conglutin immunoreactivity was slightly decreased in wheat flour mixtures compared to rice, but it was more pronounced in baked products. In meat mixtures, substantial protein fragmentation was noted after autoclaving, with decreased immunoreactivity of soybean trypsin inhibitor. The analysis of 22 commercial products enabled the identification of lupine gamma-conglutin in four bakery samples and soybean trypsin-inhibitor in five sausages, and further differentiated autoclaved from other milder thermally treated products. Generally, the immunoreactivity of target proteins was reduced by all the tested thermal treatments, though at a higher extent after autoclaving, being slightly altered by the food matrix.
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spelling pubmed-71425872020-04-15 Immunoreactivity of Lupine and Soybean Allergens in Foods as Affected by Thermal Processing Villa, Caterina Moura, Mónica B. M. V. Costa, Joana Mafra, Isabel Foods Article Lupine and soybean are important technological aids for the food industry. However, they are also capable of inducing severe allergic reactions in food-sensitized/allergic individuals. In this context, this work intended to study the combined effects of thermal processing and food matrix on the immunoreactivity of lupine and soybean proteins used as ingredients in bakery and meat products, respectively. For this purpose, the effects of baking, mild oven cooking, and autoclaving on the protein profiles were evaluated, using model mixtures simulating the production of lupine-containing breads and soybean-containing cooked hams/sausages, by native- and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and immunoblotting using specific antibodies. The results showed that lupine gamma-conglutin immunoreactivity was slightly decreased in wheat flour mixtures compared to rice, but it was more pronounced in baked products. In meat mixtures, substantial protein fragmentation was noted after autoclaving, with decreased immunoreactivity of soybean trypsin inhibitor. The analysis of 22 commercial products enabled the identification of lupine gamma-conglutin in four bakery samples and soybean trypsin-inhibitor in five sausages, and further differentiated autoclaved from other milder thermally treated products. Generally, the immunoreactivity of target proteins was reduced by all the tested thermal treatments, though at a higher extent after autoclaving, being slightly altered by the food matrix. MDPI 2020-02-27 /pmc/articles/PMC7142587/ /pubmed/32120788 http://dx.doi.org/10.3390/foods9030254 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Villa, Caterina
Moura, Mónica B. M. V.
Costa, Joana
Mafra, Isabel
Immunoreactivity of Lupine and Soybean Allergens in Foods as Affected by Thermal Processing
title Immunoreactivity of Lupine and Soybean Allergens in Foods as Affected by Thermal Processing
title_full Immunoreactivity of Lupine and Soybean Allergens in Foods as Affected by Thermal Processing
title_fullStr Immunoreactivity of Lupine and Soybean Allergens in Foods as Affected by Thermal Processing
title_full_unstemmed Immunoreactivity of Lupine and Soybean Allergens in Foods as Affected by Thermal Processing
title_short Immunoreactivity of Lupine and Soybean Allergens in Foods as Affected by Thermal Processing
title_sort immunoreactivity of lupine and soybean allergens in foods as affected by thermal processing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7142587/
https://www.ncbi.nlm.nih.gov/pubmed/32120788
http://dx.doi.org/10.3390/foods9030254
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