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Study of the AMP-activated Protein Kinase Role in Energy Metabolism Changes during the Postmortem Aging of Yak Longissimus dorsal

SIMPLE SUMMARY: Pale, soft, and exudative (PSE) meat is characterized by a pallid, sodden, and spongy appearance. Studies show that an early buildup of lactic acid due to rapid postmortem glycolysis, coupled with high muscle temperature, is the cause of PSE meat, although the precise molecular mecha...

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Autores principales: Yang, Yayuan, Han, Ling, Yu, Qunli, Gao, Yongfang, Song, Rende
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7143603/
https://www.ncbi.nlm.nih.gov/pubmed/32143283
http://dx.doi.org/10.3390/ani10030427
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author Yang, Yayuan
Han, Ling
Yu, Qunli
Gao, Yongfang
Song, Rende
author_facet Yang, Yayuan
Han, Ling
Yu, Qunli
Gao, Yongfang
Song, Rende
author_sort Yang, Yayuan
collection PubMed
description SIMPLE SUMMARY: Pale, soft, and exudative (PSE) meat is characterized by a pallid, sodden, and spongy appearance. Studies show that an early buildup of lactic acid due to rapid postmortem glycolysis, coupled with high muscle temperature, is the cause of PSE meat, although the precise molecular mechanisms remain poorly defined. We hypothesized that adenosine monophosphate activated protein kinase (AMPK) is the key factor regulating postmortem glycolysis in meat. To this end, we respectively activated and inhibited AMPK in yak muscle using AICAR and STO-609 and analyzed the metabolism parameters. The objective of this study was to establish the crucial role of AMPK in postmortem glycolysis and the possibility of targeting AMPK in order to reduce glycolysis and minimize the risk of PSE meat. Yaks are adapted to higher altitudes and lower atmospheric oxygen levels. Therefore, the activity of the yak AMPK is increased under hypoxic adaptation, which accelerates glycolysis and optimizes energy production. We further investigated the role of AMPK in the regulation of postmortem muscle glycolysis using the AMPK inhibitor STO-609 and specific activator AICAR. The objective of this study was to confirm the crucial role of AMPK in postmortem glycolysis and its potential as a target to reduce glycolysis and study of energy metabolism in yak. ABSTRACT: To explore the postmortem physiological mechanism of muscle, activity of adenosine monophosphate activated protein kinase (AMPK) as well as its role in energy metabolism of postmortem yaks were studied. In this experiment, we injected 5-amino-1-beta-d-furanonyl imidazole-4-formamide (AICAR), a specific activator of AMPK, and STO-609 to observe the changes in glycolysis, energy metabolism, AMPK activity, and AMPK gene expression (PRKA1 and PRKA2) in postmortem yaks during maturation. The results showed that AICAR could increase the expression of the PRKKA1 and PRKAA2 genes, activate AMPK and increase its activity. The effects of AICAR include a lower concentration of ATP, an increase in AMP production, an acceleration of glycolysis, an increase in the lactic acid concentration, and a decrease in the pH value. In contrast, STO-609 had the opposite effect. Under hypoxic adaptation, the activity of the meat AMPK increased, which accelerated glycolysis and metabolism and more effectively regulated energy metabolism. Therefore, this study lays the foundation for establishing a theoretical system of energy metabolism in postmortem yak meat.
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spelling pubmed-71436032020-04-14 Study of the AMP-activated Protein Kinase Role in Energy Metabolism Changes during the Postmortem Aging of Yak Longissimus dorsal Yang, Yayuan Han, Ling Yu, Qunli Gao, Yongfang Song, Rende Animals (Basel) Article SIMPLE SUMMARY: Pale, soft, and exudative (PSE) meat is characterized by a pallid, sodden, and spongy appearance. Studies show that an early buildup of lactic acid due to rapid postmortem glycolysis, coupled with high muscle temperature, is the cause of PSE meat, although the precise molecular mechanisms remain poorly defined. We hypothesized that adenosine monophosphate activated protein kinase (AMPK) is the key factor regulating postmortem glycolysis in meat. To this end, we respectively activated and inhibited AMPK in yak muscle using AICAR and STO-609 and analyzed the metabolism parameters. The objective of this study was to establish the crucial role of AMPK in postmortem glycolysis and the possibility of targeting AMPK in order to reduce glycolysis and minimize the risk of PSE meat. Yaks are adapted to higher altitudes and lower atmospheric oxygen levels. Therefore, the activity of the yak AMPK is increased under hypoxic adaptation, which accelerates glycolysis and optimizes energy production. We further investigated the role of AMPK in the regulation of postmortem muscle glycolysis using the AMPK inhibitor STO-609 and specific activator AICAR. The objective of this study was to confirm the crucial role of AMPK in postmortem glycolysis and its potential as a target to reduce glycolysis and study of energy metabolism in yak. ABSTRACT: To explore the postmortem physiological mechanism of muscle, activity of adenosine monophosphate activated protein kinase (AMPK) as well as its role in energy metabolism of postmortem yaks were studied. In this experiment, we injected 5-amino-1-beta-d-furanonyl imidazole-4-formamide (AICAR), a specific activator of AMPK, and STO-609 to observe the changes in glycolysis, energy metabolism, AMPK activity, and AMPK gene expression (PRKA1 and PRKA2) in postmortem yaks during maturation. The results showed that AICAR could increase the expression of the PRKKA1 and PRKAA2 genes, activate AMPK and increase its activity. The effects of AICAR include a lower concentration of ATP, an increase in AMP production, an acceleration of glycolysis, an increase in the lactic acid concentration, and a decrease in the pH value. In contrast, STO-609 had the opposite effect. Under hypoxic adaptation, the activity of the meat AMPK increased, which accelerated glycolysis and metabolism and more effectively regulated energy metabolism. Therefore, this study lays the foundation for establishing a theoretical system of energy metabolism in postmortem yak meat. MDPI 2020-03-04 /pmc/articles/PMC7143603/ /pubmed/32143283 http://dx.doi.org/10.3390/ani10030427 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yang, Yayuan
Han, Ling
Yu, Qunli
Gao, Yongfang
Song, Rende
Study of the AMP-activated Protein Kinase Role in Energy Metabolism Changes during the Postmortem Aging of Yak Longissimus dorsal
title Study of the AMP-activated Protein Kinase Role in Energy Metabolism Changes during the Postmortem Aging of Yak Longissimus dorsal
title_full Study of the AMP-activated Protein Kinase Role in Energy Metabolism Changes during the Postmortem Aging of Yak Longissimus dorsal
title_fullStr Study of the AMP-activated Protein Kinase Role in Energy Metabolism Changes during the Postmortem Aging of Yak Longissimus dorsal
title_full_unstemmed Study of the AMP-activated Protein Kinase Role in Energy Metabolism Changes during the Postmortem Aging of Yak Longissimus dorsal
title_short Study of the AMP-activated Protein Kinase Role in Energy Metabolism Changes during the Postmortem Aging of Yak Longissimus dorsal
title_sort study of the amp-activated protein kinase role in energy metabolism changes during the postmortem aging of yak longissimus dorsal
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7143603/
https://www.ncbi.nlm.nih.gov/pubmed/32143283
http://dx.doi.org/10.3390/ani10030427
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