Protein Engineering of a Pyridoxal-5′-Phosphate-Dependent l-Aspartate-α-Decarboxylase from Tribolium castaneum for β-Alanine Production

In the present study, a pyridoxal-5′-phosphate (PLP)-dependent L-aspartate-α-decarboxylase from Tribolium castaneum (TcPanD) was selected for protein engineering to efficiently produce β-alanine. A mutant TcPanD-R98H/K305S with a 2.45-fold higher activity than the wide type was selected through erro...

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Detalles Bibliográficos
Autores principales: Yu, Xin-Jun, Huang, Chang-Yi, Xu, Xiao-Dan, Chen, Hong, Liang, Miao-Jie, Xu, Zhe-Xian, Xu, Hui-Xia, Wang, Zhao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7143960/
https://www.ncbi.nlm.nih.gov/pubmed/32178239
http://dx.doi.org/10.3390/molecules25061280

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7143960/
https://www.ncbi.nlm.nih.gov/pubmed/32178239
http://dx.doi.org/10.3390/molecules25061280

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