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Genetic editing of the virulence gene of Escherichia coli using the CRISPR system

Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 is an emerging gene-editing technology that is widely used in prokaryotes and eukaryotes. It can realize the specific manipulation of the genome efficiently and accurately. CRISPR/Cas9 coupled λ-Red recombination technology was...

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Detalles Bibliográficos
Autores principales: Hou, Meijia, Sun, Simeng, Feng, Qizheng, Dong, Xiumei, Zhang, Ping, Shi, Bo, Liu, Jiali, Shi, Dongfang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7144585/
https://www.ncbi.nlm.nih.gov/pubmed/32292652
http://dx.doi.org/10.7717/peerj.8881