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Real-time kinetics and high-resolution melt curves in single-molecule digital LAMP to differentiate and study specific and non-specific amplification

Isothermal amplification assays, such as loop-mediated isothermal amplification (LAMP), show great utility for the development of rapid diagnostics for infectious diseases because they have high sensitivity, pathogen-specificity and potential for implementation at the point of care. However, elimina...

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Detalles Bibliográficos
Autores principales: Rolando, Justin C, Jue, Erik, Barlow, Jacob T, Ismagilov, Rustem F
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7144905/
https://www.ncbi.nlm.nih.gov/pubmed/32103255
http://dx.doi.org/10.1093/nar/gkaa099