Improved LbCas12a variants with altered PAM specificities further broaden the genome targeting range of Cas12a nucleases

The widespread use of Cas12a (formerly Cpf1) nucleases for genome engineering is limited by their requirement for a rather long TTTV protospacer adjacent motif (PAM) sequence. Here we have aimed to loosen these PAM constraints and have generated new PAM mutant variants of the four Cas12a orthologs t...

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Detalles Bibliográficos
Autores principales: Tóth, Eszter, Varga, Éva, Kulcsár, Péter István, Kocsis-Jutka, Virág, Krausz, Sarah Laura, Nyeste, Antal, Welker, Zsombor, Huszár, Krisztina, Ligeti, Zoltán, Tálas, András, Welker, Ervin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7144938/
https://www.ncbi.nlm.nih.gov/pubmed/32107556
http://dx.doi.org/10.1093/nar/gkaa110

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7144938/
https://www.ncbi.nlm.nih.gov/pubmed/32107556
http://dx.doi.org/10.1093/nar/gkaa110

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