Cargando…

Improved LbCas12a variants with altered PAM specificities further broaden the genome targeting range of Cas12a nucleases

The widespread use of Cas12a (formerly Cpf1) nucleases for genome engineering is limited by their requirement for a rather long TTTV protospacer adjacent motif (PAM) sequence. Here we have aimed to loosen these PAM constraints and have generated new PAM mutant variants of the four Cas12a orthologs t...

Descripción completa

Detalles Bibliográficos
Autores principales:	Tóth, Eszter, Varga, Éva, Kulcsár, Péter István, Kocsis-Jutka, Virág, Krausz, Sarah Laura, Nyeste, Antal, Welker, Zsombor, Huszár, Krisztina, Ligeti, Zoltán, Tálas, András, Welker, Ervin
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2020
Materias:	Molecular Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7144938/ https://www.ncbi.nlm.nih.gov/pubmed/32107556 http://dx.doi.org/10.1093/nar/gkaa110

Ejemplares similares

Mb- and FnCpf1 nucleases are active in mammalian cells: activities and PAM preferences of four wild-type Cpf1 nucleases and of their altered PAM specificity variants
por: Tóth, Eszter, et al.
Publicado: (2018)

Blackjack mutations improve the on-target activities of increased fidelity variants of SpCas9 with 5′G-extended sgRNAs
por: Kulcsár, Péter István, et al.
Publicado: (2020)

Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage
por: Kulcsár, Péter István, et al.
Publicado: (2017)

Position-dependent sequence motif preferences of SpCas9 are largely determined by scaffold-complementary spacer motifs
por: Huszár, Krisztina, et al.
Publicado: (2023)

SuperFi-Cas9 exhibits remarkable fidelity but severely reduced activity yet works effectively with ABE8e
por: Kulcsár, Péter István, et al.
Publicado: (2022)

New PAM Improves the Single-Base Specificity of crRNA-Guided LbCas12a Nuclease
por: Misiurina, Mariia A., et al.
Publicado: (2022)

A cleavage rule for selection of increased-fidelity SpCas9 variants with high efficiency and no detectable off-targets
por: Kulcsár, Péter István, et al.
Publicado: (2023)

A convenient method to pre-screen candidate guide RNAs for CRISPR/Cas9 gene editing by NHEJ-mediated integration of a ‘self-cleaving’ GFP-expression plasmid
por: Tálas, András, et al.
Publicado: (2017)

Restriction Enzyme Body Doubles and PCR Cloning: On the General Use of Type IIS Restriction Enzymes for Cloning
por: Tóth, Eszter, et al.
Publicado: (2014)

A method for characterizing Cas9 variants via a one-million target sequence library of self-targeting sgRNAs
por: Tálas, András, et al.
Publicado: (2021)

CRISPR‐LbCas12a‐mediated modification of citrus
por: Jia, Hongge, et al.
Publicado: (2019)

Cpf1 nucleases demonstrate robust activity to induce DNA modification by exploiting homology directed repair pathways in mammalian cells
por: Tóth, Eszter, et al.
Publicado: (2016)

Optimization of CRISPR/LbCas12a-mediated gene editing in Arabidopsis
por: Zhang, Qiang, et al.
Publicado: (2022)

CRISPR/LbCas12a‐mediated targeted mutation of Gracilariopsis lemaneiformis (Rhodophyta)
por: Zhang, Jingyu, et al.
Publicado: (2022)

Efficient genome editing in grapevine using CRISPR/LbCas12a system
por: Ren, Chong, et al.
Publicado: (2023)

LbCas12a mediated suppression of Cotton leaf curl Multan virus
por: Ashraf, Sidra, et al.
Publicado: (2023)

Characterization of Cas12a nucleases reveals diverse PAM profiles between closely-related orthologs
por: Jacobsen, Thomas, et al.
Publicado: (2020)

Improvement of the LbCas12a-crRNA System for Efficient Gene Targeting in Tomato
por: Vu, Tien Van, et al.
Publicado: (2021)

Efficient gene targeting in Nicotiana tabacum using CRISPR/SaCas9 and temperature tolerant LbCas12a
por: Huang, Teng‐Kuei, et al.
Publicado: (2021)

Engineered CRISPR-Cas9 nucleases with altered PAM specificities
por: Kleinstiver, Benjamin P., et al.
Publicado: (2015)

Engineering CRISPR/LbCas12a for highly efficient, temperature‐tolerant plant gene editing
por: Schindele, Patrick, et al.
Publicado: (2019)

Expanded targeting scope of LbCas12a variants allows editing of multiple oncogenic mutations
por: Choi, Eunyoung, et al.
Publicado: (2022)

Boosting genome editing efficiency in human cells and plants with novel LbCas12a variants
por: Zhang, Liyang, et al.
Publicado: (2023)

SpCas9- and LbCas12a-Mediated DNA Editing Produce Different Gene Knockout Outcomes in Zebrafish Embryos
por: Meshalkina, Darya A., et al.
Publicado: (2020)

PAM recognition by miniature CRISPR–Cas12f nucleases triggers programmable double-stranded DNA target cleavage
por: Karvelis, Tautvydas, et al.
Publicado: (2020)

Enhancing in planta gene targeting efficiencies in Arabidopsis using temperature‐tolerant CRISPR/LbCas12a
por: Merker, Laura, et al.
Publicado: (2020)

Detection of Salmonella DNA and drug-resistance mutation by PCR-based CRISPR-lbCas12a system
por: Wang, Shan, et al.
Publicado: (2023)

BEAR reveals that increased fidelity variants can successfully reduce the mismatch tolerance of adenine but not cytosine base editors
por: Tálas, András, et al.
Publicado: (2021)

Molecular basis for the PAM expansion and fidelity enhancement of an evolved Cas9 nuclease
por: Chen, Weizhong, et al.
Publicado: (2019)

Detection of Klebsiella pneumonia DNA and ESBL positive strains by PCR-based CRISPR-LbCas12a system
por: Wang, Shang, et al.
Publicado: (2023)

PEAR, a flexible fluorescent reporter for the identification and enrichment of successfully prime edited cells
por: Simon, Dorottya Anna, et al.
Publicado: (2022)

Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors
por: Huang, Tony P., et al.
Publicado: (2019)

Engineered xCas9 and SpCas9‐NG variants broaden PAM recognition sites to generate mutations in Arabidopsis plants
por: Ge, Zengxiang, et al.
Publicado: (2019)

Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for targeted mutagenesis in maize
por: Lee, Keunsub, et al.
Publicado: (2018)

Genome editing in rice mediated by miniature size Cas nuclease SpCas12f
por: Sukegawa, Satoru, et al.
Publicado: (2023)

Editor's cut: DNA cleavage by CRISPR RNA-guided nucleases Cas9 and Cas12a
por: Swartjes, Thomas, et al.
Publicado: (2020)

The prion protein family member Shadoo induces spontaneous ionic currents in cultured cells
por: Nyeste, Antal, et al.
Publicado: (2016)

Prion Protein Does Not Confer Resistance to Hippocampus-Derived Zpl Cells against the Toxic Effects of Cu(2+), Mn(2+), Zn(2+) and Co(2+) Not Supporting a General Protective Role for PrP in Transition Metal Induced Toxicity
por: Cingaram, Pradeep Kumar Reddy, et al.
Publicado: (2015)

Expanding the range of editable targets in the wheat genome using the variants of the Cas12a and Cas9 nucleases
por: Wang, Wei, et al.
Publicado: (2021)

Comprehensive assessment of miniature CRISPR-Cas12f nucleases for gene disruption
por: Xin, Changchang, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_4aj39i93tqa5fep9egg4clklhh