Single molecule analysis of effects of non-canonical guide RNAs and specificity-enhancing mutations on Cas9-induced DNA unwinding

Cas9 has made a wide range of genomic manipulation possible. However, its specificity continues to be a challenge. Non-canonical gRNAs and new engineered variants of Cas9 have been developed to improve specificity, but at the cost of the on-target activity. DNA unwinding is a checkpoint before cleav...

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Detalles Bibliográficos
Autores principales: Okafor, Ikenna C, Singh, Digvijay, Wang, Yanbo, Jung, Minhee, Wang, Haobo, Mallon, John, Bailey, Scott, Lee, Jungjoon K, Ha, Taekjip
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2019
Materias:
RNA and RNA-Protein Complexes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7145707/
https://www.ncbi.nlm.nih.gov/pubmed/31713616
http://dx.doi.org/10.1093/nar/gkz1058

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7145707/
https://www.ncbi.nlm.nih.gov/pubmed/31713616
http://dx.doi.org/10.1093/nar/gkz1058

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