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Design of a Functionalized Metal–Organic Framework System for Enhanced Targeted Delivery to Mitochondria
[Image: see text] Mitochondria play a key role in oncogenesis and constitute one of the most important targets for cancer treatments. Although the most effective way to deliver drugs to mitochondria is by covalently linking them to a lipophilic cation, the in vivo delivery of free drugs still consti...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical
Society
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146860/ https://www.ncbi.nlm.nih.gov/pubmed/32182066 http://dx.doi.org/10.1021/jacs.0c00188 |
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author | Haddad, Salame Abánades Lázaro, Isabel Fantham, Marcus Mishra, Ajay Silvestre-Albero, Joaquin Osterrieth, Johannes W. M. Kaminski Schierle, Gabriele S. Kaminski, Clemens F. Forgan, Ross S. Fairen-Jimenez, David |
author_facet | Haddad, Salame Abánades Lázaro, Isabel Fantham, Marcus Mishra, Ajay Silvestre-Albero, Joaquin Osterrieth, Johannes W. M. Kaminski Schierle, Gabriele S. Kaminski, Clemens F. Forgan, Ross S. Fairen-Jimenez, David |
author_sort | Haddad, Salame |
collection | PubMed |
description | [Image: see text] Mitochondria play a key role in oncogenesis and constitute one of the most important targets for cancer treatments. Although the most effective way to deliver drugs to mitochondria is by covalently linking them to a lipophilic cation, the in vivo delivery of free drugs still constitutes a critical bottleneck. Herein, we report the design of a mitochondria-targeted metal–organic framework (MOF) that greatly increases the efficacy of a model cancer drug, reducing the required dose to less than 1% compared to the free drug and ca. 10% compared to the nontargeted MOF. The performance of the system is evaluated using a holistic approach ranging from microscopy to transcriptomics. Super-resolution microscopy of MCF-7 cells treated with the targeted MOF system reveals important mitochondrial morphology changes that are clearly associated with cell death as soon as 30 min after incubation. Whole transcriptome analysis of cells indicates widespread changes in gene expression when treated with the MOF system, specifically in biological processes that have a profound effect on cell physiology and that are related to cell death. We show how targeting MOFs toward mitochondria represents a valuable strategy for the development of new drug delivery systems. |
format | Online Article Text |
id | pubmed-7146860 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Chemical
Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-71468602020-04-13 Design of a Functionalized Metal–Organic Framework System for Enhanced Targeted Delivery to Mitochondria Haddad, Salame Abánades Lázaro, Isabel Fantham, Marcus Mishra, Ajay Silvestre-Albero, Joaquin Osterrieth, Johannes W. M. Kaminski Schierle, Gabriele S. Kaminski, Clemens F. Forgan, Ross S. Fairen-Jimenez, David J Am Chem Soc [Image: see text] Mitochondria play a key role in oncogenesis and constitute one of the most important targets for cancer treatments. Although the most effective way to deliver drugs to mitochondria is by covalently linking them to a lipophilic cation, the in vivo delivery of free drugs still constitutes a critical bottleneck. Herein, we report the design of a mitochondria-targeted metal–organic framework (MOF) that greatly increases the efficacy of a model cancer drug, reducing the required dose to less than 1% compared to the free drug and ca. 10% compared to the nontargeted MOF. The performance of the system is evaluated using a holistic approach ranging from microscopy to transcriptomics. Super-resolution microscopy of MCF-7 cells treated with the targeted MOF system reveals important mitochondrial morphology changes that are clearly associated with cell death as soon as 30 min after incubation. Whole transcriptome analysis of cells indicates widespread changes in gene expression when treated with the MOF system, specifically in biological processes that have a profound effect on cell physiology and that are related to cell death. We show how targeting MOFs toward mitochondria represents a valuable strategy for the development of new drug delivery systems. American Chemical Society 2020-03-17 2020-04-08 /pmc/articles/PMC7146860/ /pubmed/32182066 http://dx.doi.org/10.1021/jacs.0c00188 Text en Copyright © 2020 American Chemical Society This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited. |
spellingShingle | Haddad, Salame Abánades Lázaro, Isabel Fantham, Marcus Mishra, Ajay Silvestre-Albero, Joaquin Osterrieth, Johannes W. M. Kaminski Schierle, Gabriele S. Kaminski, Clemens F. Forgan, Ross S. Fairen-Jimenez, David Design of a Functionalized Metal–Organic Framework System for Enhanced Targeted Delivery to Mitochondria |
title | Design
of a Functionalized Metal–Organic Framework
System for Enhanced Targeted Delivery to Mitochondria |
title_full | Design
of a Functionalized Metal–Organic Framework
System for Enhanced Targeted Delivery to Mitochondria |
title_fullStr | Design
of a Functionalized Metal–Organic Framework
System for Enhanced Targeted Delivery to Mitochondria |
title_full_unstemmed | Design
of a Functionalized Metal–Organic Framework
System for Enhanced Targeted Delivery to Mitochondria |
title_short | Design
of a Functionalized Metal–Organic Framework
System for Enhanced Targeted Delivery to Mitochondria |
title_sort | design
of a functionalized metal–organic framework
system for enhanced targeted delivery to mitochondria |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7146860/ https://www.ncbi.nlm.nih.gov/pubmed/32182066 http://dx.doi.org/10.1021/jacs.0c00188 |
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