Structure-based engineering of anti-GFP nanobody tandems as ultra-high-affinity reagents for purification

Green fluorescent proteins (GFPs) are widely used in biological research. Although GFP can be visualized easily, its precise manipulation through binding partners is still burdensome because of the limited availability of high-affinity binding partners and related structural information. Here, we re...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Ziyue, Wang, Yao, Ding, Yu, Hattori, Motoyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7148334/
https://www.ncbi.nlm.nih.gov/pubmed/32277083
http://dx.doi.org/10.1038/s41598-020-62606-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7148334/
https://www.ncbi.nlm.nih.gov/pubmed/32277083
http://dx.doi.org/10.1038/s41598-020-62606-7

Ejemplares similares