Enzymatic Assembly of Overlapping DNA Fragments

Three methods for assembling multiple, overlapping DNA molecules are described. Each method shares the same basic approach: (i) an exonuclease removes nucleotides from the ends of double-stranded (ds) DNA molecules, exposing complementary single-stranded (ss) DNA overhangs that are specifically anne...

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Detalles Bibliográficos
Autor principal: Gibson, Daniel G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7149801/
https://www.ncbi.nlm.nih.gov/pubmed/21601685
http://dx.doi.org/10.1016/B978-0-12-385120-8.00015-2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7149801/
https://www.ncbi.nlm.nih.gov/pubmed/21601685
http://dx.doi.org/10.1016/B978-0-12-385120-8.00015-2

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