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Methylation Pattern of the SOCS3 and IL6R Promoters in Rheumatoid Arthritis

Interleukin-6 (IL-6) plays an essential function in the development of rheumatoid arthritis (RA), mainly through its proinflammatory effect, which may lead to joint destruction. The genes encoding IL-6 receptor (IL6R) and suppressor of cytokine signaling 3 (SOCS3) play a key role in the IL-6 signali...

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Autores principales: Cieśla, Marek, Kolarz, Bogdan, Majdan, Maria, Darmochwał-Kolarz, Dorota
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7150677/
https://www.ncbi.nlm.nih.gov/pubmed/32292581
http://dx.doi.org/10.1155/2020/8394659
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author Cieśla, Marek
Kolarz, Bogdan
Majdan, Maria
Darmochwał-Kolarz, Dorota
author_facet Cieśla, Marek
Kolarz, Bogdan
Majdan, Maria
Darmochwał-Kolarz, Dorota
author_sort Cieśla, Marek
collection PubMed
description Interleukin-6 (IL-6) plays an essential function in the development of rheumatoid arthritis (RA), mainly through its proinflammatory effect, which may lead to joint destruction. The genes encoding IL-6 receptor (IL6R) and suppressor of cytokine signaling 3 (SOCS3) play a key role in the IL-6 signaling pathway, but their epigenetic regulation remains unclear. The aim of the study was to investigate how the presence of methylation in the SOCS3 and IL6R promoters is associated with the morbidity and severity of RA. A total of 146 unrelated individuals, 122 with RA and 24 healthy controls, were enrolled in the study. All subjects were genotyped with regard to the rs4969168 and rs4969170 polymorphisms in the SOCS3 gene and the rs2228145 and rs4129267 polymorphisms in IL6R. The methylation study included 52 patients with RA and 24 healthy controls. Qualitative real-time methylation-specific PCR was used to evaluate methylation status. We found no differences between patients and healthy controls in the methylation pattern in the IL6R and SOCS3 promoter regions and in variants frequency. The methylation profiles of the SOCS3 and IL6R promoters do not support the hypothesis that the genes SOCS3 and IL6R involved in the JAK-STAT signaling pathway are epigenetically deregulated in whole blood.
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spelling pubmed-71506772020-04-14 Methylation Pattern of the SOCS3 and IL6R Promoters in Rheumatoid Arthritis Cieśla, Marek Kolarz, Bogdan Majdan, Maria Darmochwał-Kolarz, Dorota Int J Inflam Research Article Interleukin-6 (IL-6) plays an essential function in the development of rheumatoid arthritis (RA), mainly through its proinflammatory effect, which may lead to joint destruction. The genes encoding IL-6 receptor (IL6R) and suppressor of cytokine signaling 3 (SOCS3) play a key role in the IL-6 signaling pathway, but their epigenetic regulation remains unclear. The aim of the study was to investigate how the presence of methylation in the SOCS3 and IL6R promoters is associated with the morbidity and severity of RA. A total of 146 unrelated individuals, 122 with RA and 24 healthy controls, were enrolled in the study. All subjects were genotyped with regard to the rs4969168 and rs4969170 polymorphisms in the SOCS3 gene and the rs2228145 and rs4129267 polymorphisms in IL6R. The methylation study included 52 patients with RA and 24 healthy controls. Qualitative real-time methylation-specific PCR was used to evaluate methylation status. We found no differences between patients and healthy controls in the methylation pattern in the IL6R and SOCS3 promoter regions and in variants frequency. The methylation profiles of the SOCS3 and IL6R promoters do not support the hypothesis that the genes SOCS3 and IL6R involved in the JAK-STAT signaling pathway are epigenetically deregulated in whole blood. Hindawi 2020-03-31 /pmc/articles/PMC7150677/ /pubmed/32292581 http://dx.doi.org/10.1155/2020/8394659 Text en Copyright © 2020 Marek Cieśla et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Cieśla, Marek
Kolarz, Bogdan
Majdan, Maria
Darmochwał-Kolarz, Dorota
Methylation Pattern of the SOCS3 and IL6R Promoters in Rheumatoid Arthritis
title Methylation Pattern of the SOCS3 and IL6R Promoters in Rheumatoid Arthritis
title_full Methylation Pattern of the SOCS3 and IL6R Promoters in Rheumatoid Arthritis
title_fullStr Methylation Pattern of the SOCS3 and IL6R Promoters in Rheumatoid Arthritis
title_full_unstemmed Methylation Pattern of the SOCS3 and IL6R Promoters in Rheumatoid Arthritis
title_short Methylation Pattern of the SOCS3 and IL6R Promoters in Rheumatoid Arthritis
title_sort methylation pattern of the socs3 and il6r promoters in rheumatoid arthritis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7150677/
https://www.ncbi.nlm.nih.gov/pubmed/32292581
http://dx.doi.org/10.1155/2020/8394659
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