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Preparation of Puerarin Chitosan Oral Nanoparticles by Ionic Gelation Method and Its Related Kinetics

In this paper, as an active ingredient, puerarin chitosan nanoparticles (Pur-CS/TPP-NPs) are prepared by an ionic gelation method. The chitosan (CS) concentration, pH of the CS solution, sodium tripolyphosphate (TPP) concentration, stirring speed, stirring time, ultrasonic power, and dosage are used...

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Autores principales: Yan, Jie, Guan, Zhi-Yu, Zhu, Wei-Feng, Zhong, Ling-Yun, Qiu, Zhuo-Qi, Yue, Peng-Fei, Wu, Wen-Ting, Liu, Jing, Huang, Xiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7150795/
https://www.ncbi.nlm.nih.gov/pubmed/32131425
http://dx.doi.org/10.3390/pharmaceutics12030216
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author Yan, Jie
Guan, Zhi-Yu
Zhu, Wei-Feng
Zhong, Ling-Yun
Qiu, Zhuo-Qi
Yue, Peng-Fei
Wu, Wen-Ting
Liu, Jing
Huang, Xiao
author_facet Yan, Jie
Guan, Zhi-Yu
Zhu, Wei-Feng
Zhong, Ling-Yun
Qiu, Zhuo-Qi
Yue, Peng-Fei
Wu, Wen-Ting
Liu, Jing
Huang, Xiao
author_sort Yan, Jie
collection PubMed
description In this paper, as an active ingredient, puerarin chitosan nanoparticles (Pur-CS/TPP-NPs) are prepared by an ionic gelation method. The chitosan (CS) concentration, pH of the CS solution, sodium tripolyphosphate (TPP) concentration, stirring speed, stirring time, ultrasonic power, and dosage are used as single factors for investigation, and the encapsulation efficiency, drug loading capacity, particle size, and polydispersity index (PDI) are used as indicators for investigation. The optimal prescription is determined using the Box–Behnken effect surface design method. The characterization of the best formulation, which is determined via an in vitro release assay and liquid chromatography/tandem mass spectrometry (LC-MS/MS) analysis methods, is used here for pharmacokinetic studies. An in situ single-pass intestinal perfusion model is used to investigate drug absorption in the intestine. After characterization, the morphologies of the nanoparticles are intact. It can be seen from the in vitro release experiments that the equation fitted by the nanoparticles is the Higuchi model, the nanoparticle release process is very stable and without sudden release, indicating that the nanoparticles are well-released in vitro. The pharmacokinetic results and the in situ single-pass intestinal perfusion model study show that the bioavailability and absorption of Pur-CS/TPP-NPs were significantly higher than Pur. Thus, all the results show that the prepared nanoparticles can significantly improve the bioavailability of Pur, and we hope to lay the foundation for the development of new products of Pur.
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spelling pubmed-71507952020-04-20 Preparation of Puerarin Chitosan Oral Nanoparticles by Ionic Gelation Method and Its Related Kinetics Yan, Jie Guan, Zhi-Yu Zhu, Wei-Feng Zhong, Ling-Yun Qiu, Zhuo-Qi Yue, Peng-Fei Wu, Wen-Ting Liu, Jing Huang, Xiao Pharmaceutics Article In this paper, as an active ingredient, puerarin chitosan nanoparticles (Pur-CS/TPP-NPs) are prepared by an ionic gelation method. The chitosan (CS) concentration, pH of the CS solution, sodium tripolyphosphate (TPP) concentration, stirring speed, stirring time, ultrasonic power, and dosage are used as single factors for investigation, and the encapsulation efficiency, drug loading capacity, particle size, and polydispersity index (PDI) are used as indicators for investigation. The optimal prescription is determined using the Box–Behnken effect surface design method. The characterization of the best formulation, which is determined via an in vitro release assay and liquid chromatography/tandem mass spectrometry (LC-MS/MS) analysis methods, is used here for pharmacokinetic studies. An in situ single-pass intestinal perfusion model is used to investigate drug absorption in the intestine. After characterization, the morphologies of the nanoparticles are intact. It can be seen from the in vitro release experiments that the equation fitted by the nanoparticles is the Higuchi model, the nanoparticle release process is very stable and without sudden release, indicating that the nanoparticles are well-released in vitro. The pharmacokinetic results and the in situ single-pass intestinal perfusion model study show that the bioavailability and absorption of Pur-CS/TPP-NPs were significantly higher than Pur. Thus, all the results show that the prepared nanoparticles can significantly improve the bioavailability of Pur, and we hope to lay the foundation for the development of new products of Pur. MDPI 2020-03-02 /pmc/articles/PMC7150795/ /pubmed/32131425 http://dx.doi.org/10.3390/pharmaceutics12030216 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yan, Jie
Guan, Zhi-Yu
Zhu, Wei-Feng
Zhong, Ling-Yun
Qiu, Zhuo-Qi
Yue, Peng-Fei
Wu, Wen-Ting
Liu, Jing
Huang, Xiao
Preparation of Puerarin Chitosan Oral Nanoparticles by Ionic Gelation Method and Its Related Kinetics
title Preparation of Puerarin Chitosan Oral Nanoparticles by Ionic Gelation Method and Its Related Kinetics
title_full Preparation of Puerarin Chitosan Oral Nanoparticles by Ionic Gelation Method and Its Related Kinetics
title_fullStr Preparation of Puerarin Chitosan Oral Nanoparticles by Ionic Gelation Method and Its Related Kinetics
title_full_unstemmed Preparation of Puerarin Chitosan Oral Nanoparticles by Ionic Gelation Method and Its Related Kinetics
title_short Preparation of Puerarin Chitosan Oral Nanoparticles by Ionic Gelation Method and Its Related Kinetics
title_sort preparation of puerarin chitosan oral nanoparticles by ionic gelation method and its related kinetics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7150795/
https://www.ncbi.nlm.nih.gov/pubmed/32131425
http://dx.doi.org/10.3390/pharmaceutics12030216
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